Method for rapidly inducing dendrobium candidum to flower in test tube

A technology of dendrobium candidum and test tube flowering, applied in the field of plant biology, to achieve the effect of large flower shape, convenient operation and high ornamental value

Inactive Publication Date: 2016-06-08
WUHAN INST OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The plant growth inhibitor PP333 can promote flower bud initiation and flowering, but the highest flowering rate is only 20.0%

Method used

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  • Method for rapidly inducing dendrobium candidum to flower in test tube
  • Method for rapidly inducing dendrobium candidum to flower in test tube
  • Method for rapidly inducing dendrobium candidum to flower in test tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: The influence of the combination of hormones of different concentrations on the flowering induction of Dendrobium officinale test-tube plantlets

[0028] 1. Test material

[0029] The test material is the tissue culture seedling of Dendrobium candidum provided by Changzhou Shihua Flower Technology Co., Ltd.

[0030] 2. Medium preparation:

[0031] For the preparation of MS basal medium and 1 / 2 basal medium, see > (compiled by Li Mingjun. Beijing: China Agricultural Press, 1992).

[0032] 2, 4-D, 6-BA, NAA, activated carbon 、GA 3 All are commercially available. All media were added with agar powder 7g / L, sucrose 30g / L and 0.1g / L activated carbon and plant growth regulators of different concentrations, and the prepared media were adjusted to about 6.0 with 0.1mol / L NaOH. In addition to GA 3 Except for external filter sterilization, other additives are added before autoclaving, pressure 1.1kg / cm -2 And time it at 121°C for 20 minutes.

[0033] 3. Dat...

Embodiment 2

[0042] Embodiment 2: the influence of different light intensities on the flowering induction of Dendrobium officinale test-tube plantlets

[0043] Light intensity plays an important role in the regulation of plant flowering. In order to explore the effect of light intensity on the flowering of Dendrobium officinale test tube seedlings, the test tube plantlets of Dendrobium officinale were inoculated to MS+2,4-D0.5mg / L+NAA0.5mg / L In the culture medium, culture was carried out under light intensity of 1000, 2000, 3000, 4000 Lux respectively, and 10 bottles were treated for each light intensity, with 4 explants per bottle, at a temperature of 24-26°C and a humidity of 35-45%. , cultured under the condition of 14-16 hours of light every day, after 80 days, the flowering situation under different light intensities was counted, and the effect of different light intensities on the flowering induction of Dendrobium officinale test-tube plantlets was explored.

[0044] result as tabl...

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Abstract

The invention discloses a method for rapidly inducing dendrobium candidum to flower in a test tube. The method comprises the following steps: step 1, inoculating a tissue culture seedling of the dendrobium candidum to a strong seedling culture medium, culturing for 25 to 30 days at temperature of 24 to 26 DEG C and humidity of 35 to 45 percent and under light of 14 to 16 hours every day to obtain a robust dendrobium candidum seedling in the test tube; step 2, inoculating the robust dendrobium candidum seedling in the test tube of which the plant height is 3 to 5 cm long to a flower bud induction and flowering culture medium, and culturing for 70 to 90 days at temperature of 24 to 26 DEG C and humidity of 35 to 45 percent and under the light of 14 to 16 hours every day to obtain a flowering plant in the test tube. The method is simple and easy to implement; flowering time can be artificially controlled to get rid of limitation of a season on the flowering phase of the dendrobium candidum; a flowering cycle is shortened; a foundation is laid for research on a flowering mechanism of the dendrobium candidum and production and application of a flower in the test tube.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for rapidly inducing test tube flowering of Dendrobium officinale. Background technique [0002] Dendrobium officinale Kimura et Migo, also known as Tiepilan or black knot grass, is a perennial epiphytic herb of the Orchidaceae (Orchidaceae) genus Dendrobium. It is named Dendrobium officinale because of its iron-green stem. Dendrobium candidum has unique medicinal value. It contains dendrobium polysaccharides, dendrobine, dendrobium phenol, dendrobium amine, etc., as well as special phenanthrene and bibenzyl anticancer ingredients. and other effects. For thousands of years, Dendrobium officinale has been listed as a top-grade Chinese medicine along with Ganoderma lucidum, ginseng, and Cordyceps sinensis, and has been praised as "the first of the nine Chinese immortal grasses" by the Taoist health classic "Dao Zang". It is a precious and endangered medicinal material u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 阳永学杨洁祝菊红赵志远李其友张安华毕秋荣杨皓琼
Owner WUHAN INST OF AGRI SCI
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