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Bivalent inactivated vaccine to bacterium pyocyaneum for mink

A technology of mink Pseudomonas aeruginosa and inactivated vaccines, applied in multivalent vaccines, vaccines, antibacterial drugs, etc., can solve the problems of low immune cross-protection, weak immunogenicity, etc., and achieve prevention of epidemics, spread, and disease rate and mortality reduction, and the effect of reducing economic losses

Inactive Publication Date: 2016-06-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a bivalent inactivated vaccine of Pseudomonas aeruginosa mink, so as to solve the problems of weak immunogenicity of existing vaccine strains and low immune cross-protection between each serotype in the research and development of Pseudomonas aeruginosa mink inactivated vaccine question

Method used

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  • Bivalent inactivated vaccine to bacterium pyocyaneum for mink
  • Bivalent inactivated vaccine to bacterium pyocyaneum for mink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1.1 Isolation and purification method of Pseudomonas aeruginosa bacterial strain

[0020] Select mink diseased from a large mink farm suspected of hemorrhagic pneumonia in mink, aseptically collect the lungs, liver and heart blood of the sick mink and inoculate them on hexadecanetrimethylammonium bromide agar medium (NAC). Cultivate in a 37°C incubator for 16h to 18h, pick round, smooth, moist, and flat colonies for Gram staining and microscopic examination, and select Gram-negative small bacilli for streaking and pure culture.

[0021] Stratify the pure culture strains on fresh blood agar plate and NAC plate respectively, culture at 37°C for 16h~18h, choose to produce green fluorescent pigment on hexadecanetrimethylammonium bromide medium, and produce β on blood agar medium Hemolyzed small colonies were purified and cultured.

[0022] According to the biochemical identification test requirements of Pseudomonas aeruginosa, the two strains will be further purified and c...

Embodiment 2

[0053] Application of mink Pseudomonas aeruginosa type B LN03 strain and G type SD17 strain in the preparation of bivalent inactivated vaccine.

[0054] Take the Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068 and the Pseudomonas aeruginosa G type SD17 strain with the preservation number CCTCCNO: M2016069 respectively, and inoculate the culture medium after activation After culturing, the bacterial liquid is collected, inactivated by formaldehyde solution, and mixed with propolis adjuvant to prepare an inactivated propolis vaccine.

[0055] The preferred operation steps are as follows:

[0056] 2.1 Strain selection

[0057] The strains used for seedling production have stable colony morphology, bacterial characteristics, biochemical characteristics, and cultural characteristics, and are highly pathogenic to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. Good immunogenicity,...

Embodiment 3

[0098] Application of mink Pseudomonas aeruginosa type B LN03 strain and G type SD17 strain in the preparation of bivalent inactivated vaccine.

[0099] Take the Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068 and the Pseudomonas aeruginosa G type SD17 strain with the preservation number CCTCCNO: M2016069 respectively, and inoculate the culture medium after activation After culturing, the bacterial liquid is collected, inactivated by formaldehyde solution, and mixed with water adjuvant to prepare a bivalent inactivated vaccine.

[0100] The preferred operation steps are as follows:

[0101] 2.1 Strain selection

[0102] The strains used for seedling production have stable colony morphology, bacterial characteristics, biochemical characteristics, and cultural characteristics, and are highly pathogenic to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. Good immunogenicity, min...

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Abstract

The invention provides a bivalent inactivated vaccine to bacterium pyocyaneum for mink. The vaccine comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated mink bacterium pyocyaneum LN03 strain having a preservation number of CCTCC NO:M2016068 and inactivated pyocyaneum SD17 having a preservation number of CCTCC NO:M2016069. The inactivated vaccine is safe and reliable and can provide effective homologous attacking protection, strong immunity can be generated after immunization, the incidence and death rate of a vaccinated mink group can be remarkably reduced, prevalence and transmission of hemorrhagic pneumonia of mink can be effectively prevented, and economic loss of hemorrhagic pneumonia of mink for fur-bearing animal breeding can be reduced. The bivalent inactivated vaccine to bacterium pyocyaneum for mink has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and in particular relates to a bivalent inactivated vaccine of Pseudomonas aeruginosa mink. Background technique [0002] Mink hemorrhagic pneumonia, also known as mink Pseudomonas pneumonia, is an acute infectious disease that mainly occurs during the moulting season of mink from August to November every year. It is characterized by hemorrhagic pneumonia and acute death. Nostrils flowed out of red foamy liquid and other symptoms. Post-mortem examination showed that the entire lung lobe was enlarged with diffuse hemorrhage and sepsis. The disease occurs all over the world and can cause 20%-40% mortality in farms every year. It is an important bacterial infectious disease that endangers the fur animal breeding industry. [0003] The disease pathogen is Pseudomonas aeruginolsa (Pseudomonasaeruginolsa) in Pseudomonas (Pseudomonadaceae) Pseudomonas (Pseudomonas), also known as Pseudomona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/104A61K39/39A61P11/00A61P31/04
CPCA61K39/104A61K39/39A61K2039/521A61K2039/55505A61K2039/55588A61K2039/70
Inventor 单虎张洪亮盖春云黄娟张传美秦志华杨瑞梅秦晓冰
Owner QINGDAO AGRI UNIV
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