Preparation method of funnel-web spider cobweb/silk fibroin composite film

A technology of funnel web spider and composite silk, which is applied in the field of preparation of funnel web spider silk composite silk fibroin protein film, which can solve the problems of insufficient strength, easy breakage, and difficult hydrolysis, etc., to save resources, improve mechanical strength, and improve breaking strength Effect

Inactive Publication Date: 2016-06-08
JIANGSU ZHENYU ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: the crystallization area of ​​the silk film prepared by the current silk fibroin is relatively small, it is easy to break when the water content is extremely low, the strength is not enough when it is applied in a low humidity environment, and the compatibility of ordinary reinforcing materials is poor. The problem is to provide a method of degumming and hydrolyzing silk to prepare a silk solution, then drying to prepare a silk film, and then dissolving the spider silk in hexafluoroisopropanol, and soaking the silk film until Among the spidroin proteins, a funnel web spider silk composite silk fibroin film is prepared by plasma grafting. The strength of the silk fibroin film prepared by the invention is greatly increased and it is not easy to be hydrolyzed

Method used

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Examples

Experimental program
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Effect test

example 1

[0019] First collect waste silk, place it in a solution with a papain concentration of 5g / L, make it completely submerged, add dropwise citric acid solution to adjust the pH to 5.5, control the temperature at 45°C, and immerse and degumming for 55 minutes. After the degumming is completed, Take out the silk, rinse twice with hot water at 90°C, hot water at 60°C and ice water at 0°C respectively, and then dry it at 100°C for 6 hours to prepare degummed silk; 25 parts of deionized water, 35 parts of calcium chloride and 40 parts of absolute ethanol were stirred and mixed to prepare the silk dissolving base liquid, and then the degummed silk prepared above was added to the In the silk dissolving base liquid, heat it in a constant temperature water bath at 75°C to dissolve it completely, then filter it with suction and collect the filtrate to prepare the silk dissolving liquid; the mass ratio of glutaraldehyde and silk dissolving liquid is 1:10, Add 0.1% glutaraldehyde to the silk...

example 2

[0021] First collect waste silk, place it in a solution with a papain concentration of 5g / L, make it completely submerged, add dropwise citric acid solution to adjust the pH to 5.7, control the temperature at 47°C, and immerse and degumming for 57 minutes. After the degumming is completed, Take out the silk, rinse twice with hot water at 90°C, hot water at 60°C and ice water at 0°C respectively, and then dry it at 102 for 7 hours to prepare degummed silk; 30 parts of deionized water, 35 parts of calcium chloride and 35 parts of absolute ethanol were stirred and mixed to obtain a silk dissolving base liquid, and then the degummed silk prepared above was added to the In the silk dissolving base liquid, heat it in a constant temperature water bath at 77°C to dissolve it completely, then filter it with suction and collect the filtrate to prepare the silk dissolving liquid; the mass ratio of glutaraldehyde and silk dissolving liquid is 1:10, Add 0.1% glutaraldehyde to the silk solu...

example 3

[0023]First collect waste silk, place it in a solution with a papain concentration of 5g / L, make it completely submerged, add dropwise citric acid solution to adjust the pH to 6.0, control the temperature at 50°C, and immerse and degumming for 60 minutes. After the degumming is completed, Take out the silk, wash it three times with hot water at 90°C, hot water at 60°C and ice water at 0°C respectively, and then dry it at 105 for 8 hours to prepare degummed silk; 45 parts of deionized water, 30 parts of calcium chloride and 25 parts of absolute ethanol were stirred and mixed to obtain a silk dissolving base liquid, and then the degummed silk prepared above was added to the In the silk dissolving base liquid, heat it in a constant temperature water bath at 80°C to dissolve it completely, then filter it with suction and collect the filtrate to prepare the silk dissolving liquid; the mass ratio of glutaraldehyde and silk dissolving liquid is 1:10, Add 0.1% glutaraldehyde to the ab...

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Abstract

The invention relates to a preparation method of a funnel-web spider cobweb/silk fibroin composite film, belonging to the field of silk fibroin film modification. The method aims to solve the problem of fewer crystalline regions, high breaking tendency under extremely low water content conditions, insufficient strength in a low-humidity environment, and poor compatibility with the common reinforcer in the silk fibroin film at present. The preparation method comprises the following steps: degumming silk, hydrolyzing to prepare a silk dissolved solution, drying to prepare a fibroin film, dissolving cobwebs in hexafluoroisopropanol, immersing the fibroin film in the cobweb protein in a plasma air atmosphere, and carrying out plasma grafting to prepare the funnel-web spider cobweb/silk fibroin composite film. The mechanical strength of the funnel-web spider cobweb/silk fibroin composite film is greatly enhanced, the fracture strength is enhanced by 30-35%, and the initial modulus is enhanced by 35-40%. The funnel-web spider cobweb/silk fibroin composite film is prepared by fully utilizing the silk and cobwebs, thereby saving the resources and being green and environment-friendly.

Description

technical field [0001] The invention relates to a method for preparing a funnel web spider silk composite silk fibroin film, which belongs to the field of silk fibroin film modification. Background technique [0002] Silk is a natural fiber, known as the "fiber queen", and has a wide range of applications in the field of clothing. Silk fibroin is a non-physiologically active natural polymer material obtained after silk degumming. It is the main body of silk, accounting for about 70% to 80% of silk mass, and its cell adhesion ability is far inferior to natural polymers. Compared with other natural polymers, silk fibroin has obvious advantages. It has a unique molecular structure, excellent moisture absorption and moisturizing properties, good biocompatibility, non-toxic, non-polluting, non-irritating, Biodegradability and antimicrobial properties; and it has been found that silk fibroin has a special affinity with human body. It is used in biosensing, biomedical materials, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00
CPCC08J5/18C08J2389/00C08J2489/00C08L89/00C08L2205/025
Inventor 李振宇高力群
Owner JIANGSU ZHENYU ENVIRONMENTAL PROTECTION TECH
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