Method for extracting mycosubtilin through separation and purification of myxococcus fulvus JCH-04 secondary metabolite

Abstract

The invention discloses a method for extracting mycosubtilin through separation and purification of myxococcus fulvus JCH-04 secondary metabolite. Myxococcus fulvus JCH-04 is preserved in the China General Microbiological Culture Collection Center. A seed medium in a shake flask is inoculated with the strain to obtain a seed solution through culture; a fermentation medium in a triangular flask is inoculated with the seed solution to obtain fermentation liquor through culture; the fermentation liquor is filtered, resin is collected and placed in a chromatographic column, and a crude extract is obtained through concentration of eluent; water and ethyl acetate are added to the crude extract to be evenly mixed and then centrifuged, aqueous phase is absorbed, and freeze-dried powder is obtained through drying; the freeze-dried powder is dissolved in methyl alcohol and centrifuged, supernate concentration is conducted, chromatographic separation is conducted with Sephadex LH-20, and fraction at 140-180 min is collected; fraction concentration is conducted, and components with retention time of 39.159 min and 42.919 min, namely C14 Mycosubtilin and C15 Mycosubtilin, are collected through semi-preparative chromatographic separation. The method is simple, mycosubtilin is extracted through separation and purification of myxococcus fulvus JCH-04 secondary metabolite, and a novel antimicrobial lipopeptides extraction approach is provided.

Description

technical field [0001] The invention relates to Myxococcus aurantiacus JCH-04, in particular to a method for separating, purifying and extracting mycosubtilin from secondary metabolites of Myxococcus aurantiacus JCH-04. Background technique [0002] Antimicrobial lipopeptides have a new antibacterial mechanism different from conventional antibiotics, not only are not easy to produce drug resistance, but also have good bactericidal and antibacterial effects on strains that have become resistant to traditional drugs; Good effect, no residue, good for G — , G + Bacteria, fungi, and mycoplasma all have good inhibitory and killing effects. With the launch of a new generation of lipopeptide antibiotics such as daptomycin and echinocandomycin, more and more researchers have turned their attention to the The research and development of this type of antibacterial substance makes it the research and development direction of green, safe, new and efficient antibacterial drugs. [000...

AI Technical Summary

Problems solved by technology

[0003] Antimicrobial lipopeptides are commonly found in the secondary metabolites of Bacillus amyloliquefaciens and Bacillus thuringiensis, and myxobacteria, as producers of natural active substances, are an important class of drug source bacteria, which can produce abnormally abundant secondary metabolites. Including aromatics, heterocycles, quinones, macrocycles, polyethers, polyenes, peptides, etc., but there is no report that it can produce antibacterial lipopeptides

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