Method for identifying cell sorting efficiency of different immunomagnetic bead cell separators

Abstract

The invention discloses a method for identifying the cell sorting efficiency of different immunomagnetic bead cell separators. Lymphocyte CD3 antibodies marked with immunomagnetic beads serve as indexes, the percentage content of CD3+ positive cells is detected and analyzed with a flow cytometry, the relative percentage is worked out with a normalization method, the separation effects of the magnetic separators with different intensities for sorting the blood lymphocyte of multiple persons are compared, and thus the purpose of selecting the separator with the optimal magnetic field for sorting various cells is achieved. Due to the fact that the whole blood lymphocyte is wide in source, the method of detecting CD3+ cells with the flow cytometry is easy and convenient to implement; CD3+ is sorted with the immunomagnetic beads, operation is easy, and the cell separating capabilities of the immunomagnetic bead cell separators with different magnetic field intensities can be conveniently and quickly identified. A quick, convenient and effective selecting method can be provided for the immunomagnetic bead separator for sorting cells.

Description

technical field [0001] The invention relates to the identification of immunomagnetic bead separators, in particular to a method for identifying the sorting efficiency of different immune magnetic bead separators by using flow cytometry to detect CD3+ cells. Background technique [0002] Immunomagnetic bead separation technology is a new immunological technology that combines the high specificity of immunological reactions with the unique magnetic responsiveness of magnetic beads. Antibody is combined to form an antigen-antibody-magnetic bead immune complex, which has high magnetic responsiveness and moves directional under the action of an external magnet to separate the complex from other substances, thereby achieving separation, concentration, The purpose of purifying microorganisms or specific antigenic substances. This technology has broad application prospects in cell separation, protein, immunology and microbiology detection. [0003] The purity and yield of immunoma...

AI Technical Summary

Problems solved by technology

The magnetic field strength of Miltenyi’s magnet is generally around 200mt. The magnetic field strength is amplified by the external separation column, so that microbeads with a diameter of 50nm are attached to the magnetic column, and the separation effect is achieved through elution; cells do not need to dissociate magnetic beads Subsequent experiments can be carried out, but the state of the sorted cells is not good

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