Method for rapidly detecting enterobacter sakazakii
A rapid technology of Enterobacter sakazakii, applied in the field of microbial detection, can solve the problems of limited optical signal of colloidal gold, limited application, low sensitivity of colloidal gold immunochromatography test strips, etc., to improve detection sensitivity, reduce workload, Uniformity of immunological response
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Embodiment 1
[0039] Embodiment one: using Fe 3 o 4 / Ru(bqy) 3 2+ Detection of Enterobacter sakazakii in milk by nano-microsphere immunochromatographic test strip
[0040] 1. Preparation of Fc-conjugated mAbs 3 o 4 / Ru(bqy) 3 2+ Nanospheres
[0041] 1.1 Fe 3 o 4 / Ru(bqy) 3 2+ Preparation of nanospheres: adding 0.6mmolFeCl 3 ·6H 2 O and 0.3 mmol FeCl 2 4H 2 O into 100mL of deionized water, nitrogen gas was passed into the solution and heated to 90°C, and then 4.7mL of 25% NH 3 ·H 2 O was added to the mixture and reacted for 2h. Use a permanent magnet to separate the black solid matter from the reaction solution and wash it with high-purity water for 3 to 5 times to obtain Fe 3 o 4 Nanoparticles; take 12mg Fe 3 o 4 Nanoparticles were resuspended with a mixture of 3mL deionized water and 20mL ethanol, and 0.5mL of NH 4 OH solution, then dissolve 50uL tetraethyl orthosilicate in 50uL ethanol solution and add dropwise, react at room temperature for 12h, in Fe 3 o 4 A laye...
Embodiment 2
[0051] Embodiment two: using Fe 3 o 4 / Ru(bqy) 3 2+ Detection of Enterobacter sakazakii in beef by nano-microsphere immunochromatographic test strip
[0052] 1. Preparation of Fc-conjugated mAbs 3 o 4 / Ru(bqy) 3 2+ Nanospheres
[0053] 1.1Fe 3 o 4 / Ru(bqy) 3 2+ Preparation of nanospheres: adding 0.6mmolFeCl 3 ·6H 2 O and 0.3 mmol FeCl 2 4H 2 O into 100mL of deionized water, nitrogen gas was passed into the solution and heated to 90°C, and then 4.7mL of 25% NH 3 ·H 2 O was added to the mixture and reacted for 2h. Use a permanent magnet to separate the black solid matter from the reaction solution and wash it with high-purity water for 3 to 5 times to obtain Fe 3 o 4 Nanoparticles; take 12mg Fe 3 o 4 Nanoparticles were resuspended with a mixture of 3mL deionized water and 20mL ethanol, and 0.5mL of NH 4 OH solution, then dissolve 50uL tetraethyl orthosilicate in 50uL ethanol solution and add dropwise, react at room temperature for 12h, in Fe 3 o 4 A layer...
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