Glycosylated protein based on soluble soybean polysaccharides and preparation method thereof

A technology of glycosylated protein and soybean polysaccharide, which is applied in the field of food processing, can solve the problem of not being able to achieve complete solubility of glycosylated protein, and achieve good market prospects, improve functional properties, and excellent dispersion stability.

Inactive Publication Date: 2016-06-15
SOUTH CHINA UNIV OF TECH
View PDF1 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, research on protein glycosylation is also a research hotspot in the food industry. At present, the polysaccharides involved in this research at home and abroad are neutral polysaccharides such as dextran, and other small molecule polysaccharides, which have not achieved glycosylation. Ideal for fully soluble proteins under acidic conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glycosylated protein based on soluble soybean polysaccharides and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Dissolve 6.25 g of soybean protein isolate (made in the laboratory, protein content is 80%) and 5 g of soluble soybean polysaccharide (Fujian Weibo Food Co., Ltd.) in deionized water, stir for 1 hour, and adjust with 0.1N HCl and 0.1N NaOH The pH value of the solution was 6.8, and a protein stock solution and a polysaccharide stock solution with a mass fraction of 10% were obtained respectively, and the two were stirred for 2 hours to mix evenly, then hydrated overnight, and then freeze-dried to obtain the mixture;

[0027] (2) reacting the freeze-dried mixture in a desiccator with a temperature of 60° C. and a humidity of 79% for 7 days to obtain a reaction product;

[0028] (3) Dissolve the reaction product obtained in step (2) in 20 times the mass of deionized water, stir for 2 hours, adjust the pH of the solution to 4.8 with 0.1N HCl, then centrifuge the solution at 10,000 rpm for 15 minutes at room temperature, and freeze the supernatant Drying yields glycosyla...

Embodiment 2

[0035] (1) Dissolve 16.67g of whey protein (made in the laboratory, protein content is 90%) and 15g of soluble soybean polysaccharide (Fujian Weibo Food Co., Ltd.) in deionized water, stir for 2h, and adjust with 0.1NHCl and 0.1NNaOH The pH value of the solution was 7.0, and a protein stock solution and a polysaccharide stock solution with a mass fraction of 10% were respectively obtained, and the two were stirred for 2 hours to mix evenly, then hydrated overnight, and then freeze-dried to obtain the mixture;

[0036] (2) reacting the freeze-dried mixture in a desiccator with a temperature of 60° C. and a humidity of 79% for 7 days to obtain a reaction product;

[0037] (3) Dissolve the reaction product obtained in step (2) in 20 times the mass of deionized water, stir for 2 hours, adjust the pH of the solution to 4.6 with 0.1N HCl, then centrifuge the solution at 10,000 rpm for 15 minutes at room temperature, and spray the supernatant Drying yields glycosylated proteins based...

Embodiment 3

[0039] (1) Dissolve 6.25g of soybean protein isolate (made in the laboratory, protein content is 80%) and 15g of soluble soybean polysaccharide (Fujian Weibo Food Co., Ltd.) in deionized water, stir for 2h, and adjust with 0.1NHCl and 0.1NNaOH The pH value of the solution was 7.0, and a protein stock solution and a polysaccharide stock solution with a mass fraction of 10% were respectively obtained, and the two were stirred for 2 hours to mix evenly, then hydrated overnight, and then freeze-dried to obtain the mixture;

[0040] (2) reacting the freeze-dried mixture in a desiccator with a temperature of 60° C. and a humidity of 79% for 10 days to obtain a reaction product;

[0041] (4) Dissolve the reaction product obtained in step (2) in 20 times the mass of deionized water, stir for 2 hours, adjust the pH value of the solution to 5.0 with 0.1N HCl, then centrifuge the solution at 10,000 rpm for 15 minutes at room temperature, and vacuum the supernatant Drying yields glycosyla...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of food processing, and discloses a glycosylated protein based on soluble soybean polysaccharide and a preparation method thereof. The preparation method is as follows: respectively dissolving protein and soluble soybean polysaccharide in deionized water, adjusting the pH value to 6.8-7.0, obtaining protein stock solution and polysaccharide stock solution respectively, mixing the two, hydrating overnight, and then freeze-drying to obtain Mixture; the freeze-dried mixture is reacted in a desiccator with a temperature of 60°C and a humidity of 79% for 7 to 14 days to obtain a reaction product, which is then dissolved in deionized water, and the pH value is adjusted to 4.6 to 4.6 after stirring and mixing. 5.0, centrifuge the solution, take the supernatant and dry to obtain glycosylated protein based on soluble soybean polysaccharide. The grafted product of the invention can effectively improve the functional properties of the protein near the isoelectric point, especially the emulsifying property, thermal stability and foaming property, which are better than the mixing effect of the two, and has a good market prospect.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a glycosylated protein based on soluble soybean polysaccharide and a preparation method thereof. Background technique [0002] The glycosylation of proteins and polysaccharides based on the Maillard reaction mechanism is a green and effective method for chemical modification of proteins, which is widely used in the food industry because of its high degree of grafting and safety. Protein and polysaccharide are the two most important types of biomacromolecules that coexist in the food emulsification system, and are the main factors affecting the structure and texture of food. Proteins are often used as emulsifiers in colloidal systems because they can form an adsorption layer on the liquid-liquid or gas-liquid interface to reduce interfacial tension. Polysaccharides are often used as stabilizers due to their good thickening and water-holding properties, thus end...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/107C07K14/415C07K14/76
CPCC07K1/1077C07K14/415C07K14/76
Inventor 齐军茹曹静
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap