Method for extracting fish oil of tilapia wastes
A technology for scraps and tilapia, which is applied in the recovery of fat oil/fatty acid, fat oil/fat production, fat oil/fat refining and other directions from waste, can solve the problems of oxidative deterioration, environmental pollution, waste of resources, etc., and achieves low cost, The effect of good quality fish oil and mild reaction conditions
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Embodiment 1
[0019] (1) After removing debris such as the fish head, fish tail, viscera of tilapia, clean them up, grind them with a meat grinder, and form a homogenate of the leftovers;
[0020] (2) Prepare the leftovers homogenate and water at a weight ratio of 1:1, heat to 35°C, keep the temperature at a constant temperature for 20 minutes, then add it to the enzymolysis tank, and raise the temperature to 40°C. The weight of tilapia leftovers is Standardly add 0.5% neutral protease, adjust to keep the pH at 7.0, stir at a constant speed of 300r / min, keep warm for 30 minutes, and inactivate the enzyme at 90°C for 15 minutes;
[0021] (3) Centrifuge the enzymolysis solution at 5000r / min for 10 minutes while it is hot. After centrifugation, the enzymolysis solution is divided into three layers, which are the enzymolysis solution in the lower layer, the insoluble matter in the middle layer, and the fish oil in the upper layer. The fish oil is obtained by absorbing the upper layer.
Embodiment 2
[0023] (1) After removing debris such as the fish head, fish tail, viscera of tilapia, clean them up, grind them with a meat grinder, and form a homogenate of the leftovers;
[0024] (2) The weight ratio of leftovers homogenate to water is 1:1.5 for deployment, heated to 36°C, kept at a constant temperature for 25min, then added to the enzymolysis tank, the temperature rises to 43°C, and the weight of tilapia leftovers is Standardly add 0.7% papain, adjust to keep the pH at 7.2, stir at a constant speed of 300r / min, keep warm for 35 minutes, and inactivate the enzyme at 95°C for 13 minutes;
[0025] (3) Centrifuge the enzymolysis liquid at 5000r / min for 12 minutes while it is hot. After centrifugation, the enzymolysis liquid is divided into three layers, which are the enzymatic hydrolysis liquid in the lower layer, the content in the middle layer and the fish oil in the upper layer. The fish oil is obtained by absorbing the upper layer.
Embodiment 3
[0027] (1) After removing debris such as the fish head, fish tail, viscera of tilapia, clean them up, grind them with a meat grinder, and form a homogenate of the leftovers;
[0028] (2) The weight ratio of leftovers homogenate to water is 1:2 for deployment, heated to 37°C, and kept at a constant temperature for 30 minutes, then added to the enzymolysis tank, the temperature rises to 45°C, and the weight of tilapia leftovers is Add 0.9% neutral protease as standard, adjust to keep the pH at 7.5, stir at a constant speed of 300r / min, keep warm for 40 minutes, and inactivate the enzyme at 98°C for 10 minutes;
[0029] (3) Centrifuge the enzymatic hydrolysis solution at 5000r / min for 15 minutes while it is hot. After centrifugation, the enzymatic hydrolysis solution is divided into three layers, namely the lower enzymatic hydrolysis solution, the middle layer containing substances and the upper layer fish oil. The fish oil is obtained by absorbing the upper layer.
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