Reagents and real-time fluorescent PCR (polymerase chain reaction) for Dysmicoccus neobrevipes (Beardsley)) detection

Abstract

The invention discloses reagents and real-time fluorescent PCR (polymerase chain reaction) for Dysmicoccus neobrevipes (Beardsley)) detection. The reagents for Dysmicoccus neobrevipes (Beardsley)) comprise a pair of primers and a probe, wherein the sequences of the forward primer and reverse primer are respectively disclosed as Seq ID No:1 and Seq ID No:2; and the sequence of the probe is disclosed as Seq ID No:3. The reagents and real-time fluorescent PCR for Dysmicoccus neobrevipes (Beardsley)) detection can substitute the traditional morphological characteristic identification method, and provide a new way for detection and identification of Dysmicoccus neobrevipes (Beardsley)). The primers and probe have the advantages of high specificity, high sensitivity, favorable repetitiveness, accurate detection and identification result and short period. The reagents and real-time fluorescent PCR overcome the defects of long detection period, demands for detection personnel with abundant experience and the like in the morphological characteristic identification. The detection reagents and real-time fluorescent PCR are especially suitable for seaport quarantine inspection, field investigation and other occasions with especially high requirements for timeliness.

Description

technical field [0001] The application relates to the field of nucleic acid molecule detection, in particular to a reagent and real-time fluorescent PCR for the detection of Pneumococcus chinensis. Background technique [0002] New pineapple mealybug (Dysmicoccus neobrevipes (Beardsley)) has been included in the "List of Dangerous Diseases, Pests and Weeds for Imported Plant Quarantine of the People's Republic of China" announced by my country in May 2007. It is an important pest of great economic significance and international concern. . The new pineapple mealybug mainly harms pineapple, banana, custard apple, citrus, grapes and other plants. Eggs, larvae and adults are all parasitic on the surface of host plants, and can transmit pineapple wilt virus (PMEaV), causing malnutrition in host plants, tree Weakness, a large number of leaf and fruit drop, and severe plant death. Pneumococcus neopineapple is mostly distributed in tropical countries and regions where bromeliad pla...

AI Technical Summary

Problems solved by technology

At present, the research and reports on the application of the real-time fluorescent PCR detection method of TaqManMGB probes to the rapid detection of insects are still relatively lacking, and more exploratory research work is needed

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