Method and kit for detecting progesterone and testosterone in saliva through high performance liquid chromatography-tandem mass spectrometry technique

A technology of high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of detection of progesterone and testosterone in saliva by high performance liquid chromatography and tandem mass spectrometry, achieving the effect of simple operation, short analysis time and good reproducibility
CN105675788AActive Publication Date: 2016-06-15北京洛奇医学检验实验室股份有限公司
5 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京洛奇医学检验实验室股份有限公司
Publication Date
2016-06-15

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method for detecting progesterone and testosterone in saliva through a high performance liquid chromatography-tandem mass spectrometry technique.The method comprises the steps that the progesterone and the testosterone in pretreated saliva are detected by adopting the high performance liquid chromatography-tandem mass spectrometry technique, the progesterone and the testosterone are separated from impurities through high performance liquid chromatography, quantification is performed through an internal standard method, an isotope of the progesterone is taken as an internal standard, a calibration curve is built by taking the concentration ratio of a standard product to the internal standard as an X axis and taking the peak area ratio of the standard product to the internal standard as a Y axis, and the content of the progesterone and the testosterone is calculated.The method is high in sensitivity and specificity, good in accuracy and simple in pretreatment process; the analysis time is short, analysis of the progesterone and the testosterone can be completed in 6.5 min, and the precision degree and the standard recovery rate meet the detection requirement.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of saliva detection, in particular to a method for detecting progesterone and testosterone in saliva by high performance liquid chromatography tandem mass spectrometry. Background technique

[0002] At present, the commonly used methods for detecting progesterone and testosterone in saliva include enzyme-linked immunoassay, electrochemiluminescence immunoassay, etc. These methods are affected by various objective conditions, such as temperature, pH value, ionic strength, and reagent immune activity. Immunoassay is unavoidable due to the interference of structurally similar substances in the saliva matrix, and the sensitivity is low. The test results of kits produced by different manufacturers have large deviations, and the lack of uniform standards leads to large deviations in test results and low reference value. Contents of the invention

[0003] The purpose of the present invention is to solve some pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More