Sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle
A technology for p-acetophenone and secondary metabolites, applied in the directions of animal repellents, botanical equipment and methods, chemicals for biological control, etc., can solve the unreported secondary metabolites of alder The composition analysis, the natural growth environment and tending time of restricted non-host forest trees, the unclear composition of secondary metabolites and active components, etc., can achieve the effect of curbing harm and spreading, convenient and quick promotion and application, and saving labor and capital costs.
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[0032] 1 Materials and methods
[0033] 1.1 Screening and identification of secondary metabolites
[0034] 1.1.1 Collection and analysis of non-host plant secondary metabolites
[0035] The secondary metabolites of Alnus sichuan-yunnanensis were collected by headspace dynamic method. The collected leaves and branches were wrapped in an odorless transparent plastic bag (PTEK), with an air inlet and an air outlet at both ends of the bag. The air at the inlet end is filtered by activated carbon (ORBOTM-32), and the flow rate is controlled at 400mL / min; the air outlet end flows through 150mg PorapakQ adsorbent (50-80mesh), the flow rate is 300mL / min, and the collection time is 1.5h. After the adsorption is complete, it is eluted with n-hexane (HPLC), and 300 μL of the eluate is stored in a brown reagent bottle with a capacity of 2 mL. Then via N 2 Concentrate to 100μL, add n-octane (0.702g / cm 3 ) and decyl acetate (0.865g / cm 3 ) two internal standard compounds, each internal...
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