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Sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle

A technology for p-acetophenone and secondary metabolites, applied in the directions of animal repellents, botanical equipment and methods, chemicals for biological control, etc., can solve the unreported secondary metabolites of alder The composition analysis, the natural growth environment and tending time of restricted non-host forest trees, the unclear composition of secondary metabolites and active components, etc., can achieve the effect of curbing harm and spreading, convenient and quick promotion and application, and saving labor and capital costs.

Inactive Publication Date: 2016-06-29
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the composition and active components of the secondary metabolites are not clear at present. Only the compounds in Nepalese alder, Sichuan and Yunnan alder fruits, and alder leaves are seen in the literature, and these compounds are mainly high-molecular secondary metabolites. A relatively complete report on the components of secondary metabolites in Alnus sichuan-yunnan and the analysis of these components
However, alder alder in Sichuan and Yunnan grows at an altitude of 1500-3000m, with a soil layer thickness greater than 80cm, in moist yellow-red or purple soil, and the growth period is at least 5 years, which limits the effectiveness of interplanting trees to prevent damage
Relevant patent documents, such as "Rapid screening method for mixed forest model against yunnanensis bark beetle (CN201010249717.1)" is a method for cultivating non-host forest trees to build mixed forests to achieve continuous control of bark beetle, but this method is also limited by non-host forest trees Natural growth environment and rearing time

Method used

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  • Sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle
  • Sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle
  • Sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle

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Embodiment Construction

[0032] 1 Materials and methods

[0033] 1.1 Screening and identification of secondary metabolites

[0034] 1.1.1 Collection and analysis of non-host plant secondary metabolites

[0035] The secondary metabolites of Alnus sichuan-yunnanensis were collected by headspace dynamic method. The collected leaves and branches were wrapped in an odorless transparent plastic bag (PTEK), with an air inlet and an air outlet at both ends of the bag. The air at the inlet end is filtered by activated carbon (ORBOTM-32), and the flow rate is controlled at 400mL / min; the air outlet end flows through 150mg PorapakQ adsorbent (50-80mesh), the flow rate is 300mL / min, and the collection time is 1.5h. After the adsorption is complete, it is eluted with n-hexane (HPLC), and 300 μL of the eluate is stored in a brown reagent bottle with a capacity of 2 mL. Then via N 2 Concentrate to 100μL, add n-octane (0.702g / cm 3 ) and decyl acetate (0.865g / cm 3 ) two internal standard compounds, each internal...

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Abstract

Provided are a sustained-release agent and method using Alnus ferdinandi-coburgii secondary metabolite p-phenylethylketone to avoid Pinus yuannanensis pine shoot beetle. P-phenylethylketone solution is prepared using n-hexane or n-pentane or petroleum ether as a solvent and p-phenylethylketone as a solute and is used for providing avoidance for forest stands under different degrees of harm from Pinus yuannanensis pine shoot beetle; the concentration of p-phenylethylketone is positively correlated to the degree of harm to the forest stand from Pinus yuannanensis pine shoot beetle. The application refers to: setting the concentration of the sustained-release agent to 50-500 ng / MuL, putting the agent in sustained-release bottles with the top end opened, hanging the bottles in a crossing manner on weak tree shoots in a tower form according to the direction and tree height. The agent is made from the non-host forest secondary metabolite of Pinus yuannanensis pine shoot beetle, is high in avoiding capacity, long in duration, low in price and environment-friendly, is sustainably useful in controlling the species quantity of Pinus yuannanensis pine shoot beetle and has great popularization potential.

Description

technical field [0001] The invention belongs to the prevention and control of forestry pests, and relates to a drug slow-release agent for preventing and controlling the yunnanensis bark beetle. Background technique [0002] Yunnan pine beetle is a kind of stem-boring beetle characterized by damage to Yunnan pine, such as Tomicus yunnanensis, T.minor and T. brevipilosus. The beetle, newly emerged adults like to eat healthy pine shoots, and the duration of 8 to 9 months will cause the tree to weaken; after sexual maturity, it will choose the weakened Yunnan pine trunk as food, eggs and reproduction, and will last for 3 to 4 months. cause trees to die. However, the bark beetle is affected by non-host odors from the search for hosts to the encounter of male and female individuals, mating, oviposition, and post-embryonic development. It has a high ability to select and identify host plants in forest areas. [0003] Alnusferdinandi-coburgii Schneid belongs to the Alnus genus of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/02A01N25/18A01N35/04A01P17/00A01M1/20
CPCA01M1/20A01N25/02A01N25/18A01N35/04
Inventor 李宗波赵宁朱家颖杨斌王大伟泽桑梓
Owner SOUTHWEST FORESTRY UNIVERSITY
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