The secondary metabolite of Alder in Sichuan and Yunnan, p-ethylacetophenone, is a sustained-release agent and method for avoiding Pine beetle
A technology for p-ethylacetophenone, secondary metabolites, applied in the fields of animal repellants, botanical equipment and methods, chemicals for biological control, etc., can solve the natural growth environment of restricted non-host forest trees and Tending time, unreported composition analysis of secondary metabolites of alder in Sichuan and Yunnan, unclear composition and active components of secondary metabolites, etc., to achieve the effect of curbing harm and spreading, convenient and quick promotion and application, and saving labor and capital costs
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[0031] 1 Materials and methods
[0032] 1.1 Screening and identification of secondary metabolites
[0033] 1.1.1 Collection and analysis of non-host plant secondary metabolites
[0034] The secondary metabolites of Alnus sichuan-yunnanensis were collected by headspace dynamic method. The collected leaves and branches are wrapped in an odorless transparent plastic bag (PTEK), and the two ends of the bag are respectively provided with an air inlet and an air outlet. The air at the inlet end was filtered by activated carbon (ORBOTM-32), and the flow rate was controlled at 400mL / min; the air outlet end flowed through 150mg Porapak Q adsorbent (50-80mesh), at a flow rate of 300mL / min, and the collection time was 1.5h. After the adsorption was completed, it was eluted with n-hexane (HPLC), and 300 μL of the eluate was stored in a brown reagent bottle with a capacity of 2 mL. Then via N 2 Concentrate to 100μL, add n-octane (0.702g / cm 3 ) and decyl acetate (0.865g / cm 3 ) Two int...
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