Preparation method for polymer micro-fluidic chips on basis of 3D hydrogel printing

A microfluidic chip, 3D printing technology, applied in additive processing and other directions, can solve problems such as polymer microfluidic chips that have not yet been found

Active Publication Date: 2016-06-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after reviewing domestic and foreign literature, no reports have been found on the use of 3D printed hydrogel male molds to process polymer microfluidic chips.

Method used

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  • Preparation method for polymer micro-fluidic chips on basis of 3D hydrogel printing
  • Preparation method for polymer micro-fluidic chips on basis of 3D hydrogel printing
  • Preparation method for polymer micro-fluidic chips on basis of 3D hydrogel printing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Application of 3D printed carrageenan hydrogel positive mold in the preparation of polymethyl methacrylate microfluidic chip

[0042] First, it is necessary to prepare carrageenan thermoreversible hydrogel for 3D printing, which mainly contains water, cross-linking agent potassium chloride and carrageenan. The formula of carrageenan hydrogel: 1.5% carrageenan, 1% potassium chloride cross-linking agent, and the balance of water. Weigh 15 grams of carrageenan powder and disperse it into 800 grams of water, and swell at room temperature (about 25°C) for 6 hours. Then, heat to 95°C to completely dissolve the carrageenan. Dissolve 10 grams of potassium chloride in 175 grams of water, mix evenly with heated carrageenan solution, degas under negative pressure, cast into strips with a diameter of 3-8 mm, and store in a refrigerator at 4°C. The above formula was obtained through a series of optimization experiments, mainly by adjusting the concentration of hydrogel ...

Embodiment 2

[0049] Example 2, Application of 3D printed agar hydrogel positive mold in the preparation of polydimethylsiloxane microfluidic chip

[0050] The design of the microfluidic chip and the manufacturing method of the agar hydrogel positive mold are the same as in Example 1.

[0051] Mix 77 grams of water with 2.5 grams of agar powder and let the agar swell for 3 hours. Then, heat to 90°C to completely dissolve the agar. Dissolve 0.5 g of borax in 20 ml of water, mix the two solutions while hot, and cool to room temperature to obtain a 3D printing hydrogel.

[0052] as attached figure 2 As shown, before printing the agar hydrogel positive mold, the polymethyl methacrylate bottom plate 9 is cleaned and then dried, coated with a small amount of release agent (0.5% sodium stearate solution) and dried, then placed in the 3D printer Fix and adjust the level on the printing platform at the bottom. Heat the agar thermally reversible hydrogel for 3D printing to 70°C to melt, inject i...

Embodiment 3、3

[0054] Example 3, Application of 3D printed carrageenan hydrogel positive mold in the preparation of epoxy resin microfluidic chip

[0055] The design and processing of the carrageenan hydrogel positive mold for casting epoxy resin microfluidic chips are the same as in Example 1. After mixing commercial E51 epoxy resin and curing agent ethylenediamine in a mass ratio of 90:10 and vacuum degassing, the epoxy resin casting solution containing curing agent can be obtained. Take 1.5 milliliters of the casting solution 10 along the carrageenan hydrogel anode. The center line of the long side of the mold 8 is poured on it, and covered with a self-made transparent epoxy resin cover plate 11 (75mm×16mm×1.0mm). The gap between the cover plate 11 and the hydrogel male mold 8 is 0.5 mm and filled with the casting solution 10 . For the epoxy resin casting solution cast between the hydrogel male mold 8 and the transparent epoxy resin cover plate 11, it is irradiated with infrared rays 12,...

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Abstract

The invention belongs to the technical field of micro-fluidic chips and particularly discloses a preparation method for the polymer micro-fluidic chips on the basis of 3D hydrogel printing. The preparation method comprises the steps of preparing hot and reversible carrageenan or agar hydrogel, and improving the mechanical strength of the hydrogel, lowering the viscosity of the molten hydrogel and increasing the gelatinization speed after the hydrogel is cooled by adjusting the concentration of the hydrogel and using cross-linking agents; designing structural units such as channel networks, solution holes and reactors of the micro-fluidic chips through software, outputting the design documents to a high-accuracy 3D hydrogel printer and printing the hydrogel on a bottom plate for obtaining male hydrogel molds; mixing macromolecular prepolymers and curing agents for obtaining mold casting solutions, pouring the solutions on the male molds, covering the male molds with cover plates, enabling the gaps between the cover plates and the male molds to be filled with mold casting solutions, releasing the molds for obtaining micro-fluidic chip substrates after curing and obtaining finished products of the micro-fluidic chips after the substrates and cover sheets are packaged. According to the method, the polymer micro-fluidic chips made of polymethyl methacrylate, polydimethylsiloxane or epoxy resin can be processed.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a method for preparing a polymer microfluidic chip based on hydrogel 3D printing. Background technique [0002] Microfluidic chips are mainly made of glass, quartz and polymers[1]. Among them, glass and quartz microfluidic chips are usually processed by a combination of photolithography and chemical etching, which requires high technology and equipment, and it is difficult to use molds in large quantities. Production is relatively expensive, which limits its wide application. Therefore, in recent years, polymer microfluidic chips have been developed and valued, and can be mass-produced at low cost by using molds through techniques such as injection molding, impression molding, and casting [2]. The polymers used to process microfluidic chips include polymethyl methacrylate (PMMA), polydimethylsiloxane (PDMS), polycarbonate (PC), polystyrene (PS), etc. [3], ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C39/02B29C33/38B29C67/00B33Y10/00B33Y50/00C08F120/14
CPCB29C33/38B29C33/3835B29C33/3842B29C39/02B33Y10/00B33Y50/00C08F120/14
Inventor 陈刚韩毓张剑霞张鲁雁
Owner FUDAN UNIV
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