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Method for preparing agarose cross-linked gel microspheres

A cross-linked gel and agarose technology, applied in the field of polymers, can solve the problems of unfavorable column operation, high back pressure of chromatography column, and limited application, and achieve the effect of good mechanical strength, simple equipment, and uniform ball size

Active Publication Date: 2016-06-29
AMICOGEN CHINA BIOPHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the prepared beads are used, the pressure drop is large, and the back pressure of the chromatography column is high, which is not conducive to column operation and limits its application.

Method used

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  • Method for preparing agarose cross-linked gel microspheres
  • Method for preparing agarose cross-linked gel microspheres
  • Method for preparing agarose cross-linked gel microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 7.5 g of agarose in 250 mL of water (concentration of agarose aqueous solution is 3%), stir at room temperature for 1 h, add 0.12 g of KCl and continue stirring for 0.5 h, then heat until the agarose boils and the solution becomes transparent. Prepare 1.5L toluene solution, add 23.49g polyvinyl acetate and 2.349g oleic acid. Stir the organic phase at a constant temperature at 70° C. until uniformly mixed, pour the boiling agarose aqueous solution into the organic phase, and stir for 1 hour to disperse and emulsify the agarose. Add 22.5g of epichlorohydrin at one time, add 20mL of 50% NaOH solution dropwise, and complete the dropwise addition within 1 hour. Stir and solidify for 3 hours before condensing, and control the cooling rate at 2°C / min until the temperature is cooled below 10°C. Agarose gel microspheres with a particle size of 100-300 meshes are prepared after repeated washing.

Embodiment 2

[0042] Take 10 g of agarose in 250 mL of water (the concentration of the agarose aqueous solution is 4%), stir at room temperature for 1 h, add 0.2 g of KCl and continue stirring for 0.5 h, then heat until the agarose boils and the solution becomes transparent. Prepare 1.75L toluene solution, add 30.45g polyvinyl acetate and 3.045g oleic acid. Stir the organic phase at a constant temperature at 70° C. until uniformly mixed, pour the boiling agarose aqueous solution into the organic phase, and stir for 1 hour to disperse and emulsify the agarose. Add 30g of epichlorohydrin at one time, and add 25mL of 50% NaOH solution dropwise, and the dropwise addition is completed within 1 hour. Stir and solidify for 3 hours before condensing, and control the cooling rate at 2°C / min until the temperature is cooled below 10°C. Agarose gel microspheres with a particle size of 100-300 meshes are prepared after repeated washing.

Embodiment 3

[0044] Take 15 g of agarose in 250 mL of water (concentration of agarose aqueous solution is 6%), stir at room temperature for 1 h, add 0.375 g of KCl and continue stirring for 0.5 h, then heat until the agarose boils and the solution becomes transparent. Prepare 1.625L toluene solution, add 35.3g polyvinyl acetate and 3.53g oleic acid. Stir the organic phase at a constant temperature at 70° C. until uniformly mixed, pour the boiling agarose aqueous solution into the organic phase, and stir for 1 hour to disperse and emulsify the agarose. Add 45g of epichlorohydrin at one time, and add 38mL of 50% NaOH solution dropwise, and the dropwise addition is completed within 1 hour. Stir and solidify for 3 hours before condensing, and control the cooling rate at 2°C / min until the temperature is cooled below 10°C. Agarose gel microspheres with a particle size of 100-300 meshes are prepared after repeated washing.

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Abstract

The invention belongs to the filed of polymer, particularly relates to a method for preparing agarose cross-linked gel microspheres and provides microspheres which are good in mechanical property, high in heat resistance and pressure resistance and uniform in particle size. An agarose solution serves as an aqueous phase, methylbenzene serves as an oil phase, epoxy chloropropane is adopted for performing cross-linking in the forming process of agarose microspheres, and the agarose gel microspheres good in mechanical property are prepared. The invention further aims at providing the convenient and simple preparation method of the agarose cross-linked gel microspheres.

Description

technical field [0001] The invention belongs to the field of macromolecules, in particular to a preparation method of agarose cross-linked gel microspheres. Background technique [0002] Agarose Agarose, abbreviated as AG, is an uncharged neutral component in agar, also translated as agarose or agarose. Agar has a long history as a unique food in our country. It is made by extracting and separating large marine algae such as Geliflower, Porphyra, and Jiangli. Agarose can form a uniform solution when heated, which is a single polysaccharide macromolecular chain. When the temperature is lowered, a parallel double helix structure is first formed under the action of hydrogen bonds, and the double helix aggregates to form micelles with an ordered structure. A pentagonal hole is formed between them. When agarose forms a gel, it has a spatial network structure and a moderate pore size distribution. Therefore, agarose gel microspheres can be used as a medium for gel sieving and a...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/16C08L31/04C08L5/12C08K3/16C08K5/1515C08K5/09
CPCC08J3/16C08J3/24C08J2331/04C08J2405/12C08L31/04C08L2201/08C08L2312/00C08L5/12C08K3/16C08K5/1515C08K5/09
Inventor 刘书霞罗文军王玲杨申勇
Owner AMICOGEN CHINA BIOPHARM CO LTD
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