A kind of high biological activity chitin and its preparation method

Abstract

The invention provides high-bioactivity chitin and a preparing method thereof. The preparing method comprises the steps of washing, drying and smashing an organism containing beta-chitin into powder; pouring the powder obtained through smashing into mixed base solution with the concentration of 0.5-10 M to be stirred for alkali treatment deproteinization; adding diluted hydrochloric acid solution into the product obtained after alkali treatment deproteinization, regulating the pH value to be 5-7, standing at 20-30 DEG C, removing inorganic salt, and conducting filtration to obtain beta-chitin. The chitin obtained by means of the technical scheme has the advantages of being high in molecular weight, high in adsorption capacity, high in amidogen remaining quantity, excellent in adsorbability, moisture absorbability and moisture retentivity, ultrahigh in beta-chitin deacetylation degree and the like, so that the product has quite high bioactivity and can be widely applied to the field of biological materials.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a chitin with high biological activity and a preparation method thereof. Background technique [0002] The currently commercially available natural biopolymer material chitin is isolated from shrimp and crab shells, which is rich in sources. At present, there are many methods to extract chitin from shrimp and crab shells, including chemical treatment and mechanical grinding-ultrasonic-centrifugation, heterogeneous synthesis, freezing-thawing and other methods. The main component of shrimp and crab shells is chitin, which is chemically inactive, does not change with body fluids, does not react with foreign bodies to tissues, is non-toxic, and has the characteristics of anti-thrombosis and high-temperature disinfection. Chitin and its derivatives prepared from chitin have very good biocompatibility, biodegradability, antibacterial and anti-inflammatory properties, and have been...

AI Technical Summary

Problems solved by technology

[0004] 1. The yield of chitin prepared by mechanical grinding-ultrasonic-centrifugation is low;

[0005] 2. The heterogeneous method uses high-concentration lye to carry out alkalization, the molecular weight of the product is seriously degraded, and the environment is polluted;

[0006] 3. The heterogeneous method is easy to destroy the aggregate structure during the dissolution and solidification process, and the mechanical properties of the obtained chitin are weak and brittle, which limits the application of chitin as a membrane material;

[0007] 4. The preparation of chitin solution by freezing-thawing method is time-consuming and energy-consuming, and is not suitable for large-scale production;

But our research shows that when the degree of deacetylation of α-chitin exceeds 90%, because the molecular chain tends to be relatively regular, the increase of crystallinity leads to the decrease of the amino group content on the surface of solid chitin and the amorphous region, and the adsorption effect suddenly decreases.

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