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Preparation technology of fly maggot chitin and chitosan

A technology of fly maggot chitin and chitosan, applied in the biological field, can solve the problems of poor product quality, environmental pollution and high cost, and achieve the effects of low water content, shortened reaction time and high degree of deacetylation

Inactive Publication Date: 2015-01-14
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the invention is to propose a preparation process of fly maggot chitin and chitosan, which solves the problems of high cost, high energy consumption and poor product quality in the traditional process of preparing chitin and chitosan with concentrated acid and concentrated alkali , and will cause serious environmental pollution

Method used

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  • Preparation technology of fly maggot chitin and chitosan
  • Preparation technology of fly maggot chitin and chitosan
  • Preparation technology of fly maggot chitin and chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0103] (1) Raw material pretreatment

[0104] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0105] (2) EDTA decalcification

[0106] Weigh the clean and dry fly maggot skins obtained in step (1), soak them in (0.3mol / L) saturated EDTA solution for 24 hours to remove calcium, then filter to remove EDTA (recycling), and remove the maggot skins Wash with water until neutral;

[0107] (3) Dilute alkali to remove protein

[0108] Use 10% NaOH aqueous solution on the maggot skin treated in step (2) and boil water bath for 2 hours to hydrolyze the protein. Stir continuously during heating, and replace the lye every half hour. After heating, remove the lye and wash with water until neutral , to obtain crude chitin;

[0109] (4) Refin...

Embodiment 2

[0112] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0113] (1) Raw material pretreatment

[0114] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0115] (2) EDTA decalcification

[0116] Weigh the clean and dry fly maggot skins obtained in step (1), soak them in saturated EDTA solution for 20 hours, remove calcium, filter to remove EDTA (recycling), and wash the maggot skins with water until neutral;

[0117] (3) Dilute alkali to remove protein

[0118] Use 8% NaOH aqueous solution on the maggot skin treated in step (2) and boil water bath for 1.5h to hydrolyze the protein. Stir continuously during the heating period and replace the lye every half hour. properties, to obtain crude chitin;

[0119] (4) Refined finished products

[0120] The crude chitin obtained in step (3) was ...

Embodiment 3

[0122] Present embodiment is the preparation technology of fly maggot chitin, prepares according to the following steps:

[0123] (1) Raw material pretreatment

[0124] Crush the dried fly maggots, wash them with water, wash off a large amount of protein components, and filter and collect the remaining maggot skins for later use;

[0125] (2) EDTA decalcification

[0126] Weigh the clean and dry fly maggot skins obtained in step (1), soak them in saturated EDTA solution for 30 hours, remove calcium, filter to remove EDTA (recyclable reuse), and wash the maggot skins with water until neutral;

[0127] (3) Dilute alkali to remove protein

[0128] Use 12% NaOH aqueous solution on the maggot skins treated in step (2) for 3 hours in a boiling water bath to hydrolyze the protein, stir continuously during heating, and replace the lye every half an hour. After heating, remove the lye and wash with water until neutral , to obtain crude chitin;

[0129] (4) Refined finished p...

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Abstract

The invention discloses a preparation technology of fly maggot chitin and chitosan and an application of fly maggot chitin to inducing a trichoderma aureoviride T2 bacterial strain to generate chitinase, belonging to the field of biological technology. The preparation technology of fly maggot chitin has the main characteristics that the traditional hydrochloric acid calcium removal technology is changed into an EDTA (Ethylene Diamine Tetraacetic Acid) calcium removal technology, the environmental pollution caused by waste acid discharge can be reduced, the damage of concentrated acid on chitin molecular chains can be avoided, and the chitin product with the high quality is obtained; and according to the preparation technology of fly maggot chitin, the traditional technology of 4-hour high-temperature concentrated alkaline refluxing is changed into a sodium hydroxide-amyl alcohol reaction system, microwave heating treatment is carried out twice, the utilization rate of alkali liquid is increased, the reaction time is greatly shortened, the energy consumption is saved, and a white powdery chitosan product with the lower moisture content and the higher degree of deacetylation is obtained. The fly maggot chitin disclosed by the invention can be used as a carbon source for inducing the trichoderma aureoviride T2 bacterial strain to generate the chitinase and has obvious induction effect.

Description

[0001] This application is a divisional application of "a preparation process of fly maggot chitin and chitosan and its application in inducing the production of chitinase by dark green xylinase T2 strain". Its application number is 201010135626.5 and the filing date is March 30, 2010. technical field [0002] The invention relates to the field of biotechnology, in particular to a preparation process of fly maggot chitin and chitosan and its application in inducing the production of chitinase by dark green xylinase T2 strain. Background technique [0003] Chitin (Chitin) is also known as chitin, chitin, chitin, polyacetylglucosamine, etc., and its chemical name is 1,4-2-acetylamino-2-deoxy-β-D-glucan, which is N- Acetyl-D-glucosamine (GlcNAc) is a linear polymer (C 16 h 26 o 10 N 2 ) n. Widely present in the crustaceans of shrimps, crabs, insects, fungi (yeast, mold), algae and plant cell walls. Chitosan (Chitosan) is chitin off C 2 Acetyl products, also known as chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 梁巧兰徐秉良师桂英魏列新薛应钰杨顺义宋文勇
Owner GANSU AGRI UNIV
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