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Rinsing liquid for nucleic acid extraction purification

A rinsing solution and nucleic acid technology, applied in the field of rinsing solution, can solve problems such as affecting nucleic acid stability and cross-contamination, and achieve the effects of reducing the chance of cross-contamination, eliminating the inhibitory effect, and reducing production costs.

Inactive Publication Date: 2016-07-06
SUZHOU SYM BIO LIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the drying process increases the exposure time of the magnetic beads, it may affect the stability of the extracted nucleic acid. In addition, in order to speed up the volatilization of ethanol or isopropanol, some commercially available automatic extraction instruments are equipped with a fan with a heating function to make the magnetic beads Accelerated circulation of air around the beads increases the risk of aerosolization of extracted nucleic acid products and cross-contamination between samples

Method used

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  • Rinsing liquid for nucleic acid extraction purification
  • Rinsing liquid for nucleic acid extraction purification
  • Rinsing liquid for nucleic acid extraction purification

Examples

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Effect test

Embodiment 1

[0046] Embodiment 1. the application of rinse solution of the present invention in the quantitative detection of hepatitis B virus (HBV) nucleic acid

[0047] In this embodiment, the rinsing solution provided by the present invention is used to replace the rinsing solution in the commercially available HBV nucleic acid quantitative reagent, and the drying step of the rinsing solution is omitted in the nucleic acid extraction process, and the HBV international standard is quantitatively detected. From the results of the test Whether the rinsing solution provided by the present invention is judged whether it is feasible in terms of accuracy and sensitivity, the specific research materials are as follows:

[0048] (1) "Hepatitis B Virus Nucleic Acid Detection Kit (Fluorescent Quantitative PCR Method)" produced by Shanghai Haoyuan Biotechnology Co., Ltd. (Medical Device Registration Certificate Number: National Food and Drug Administration (Zhun) No. 2013 No. 3402077); This produc...

Embodiment 2

[0075] Embodiment 2 Application of the rinsing solution of the present invention in the quantitative detection of hepatitis C virus (HCV) nucleic acid

[0076]In this embodiment, the rinsing solution provided by the present invention is used to replace the rinsing solution in the commercially available HCV nucleic acid quantitative reagent, and the drying step of the rinsing solution is omitted in the nucleic acid extraction process to carry out quantitative detection on the HCV international standard. Judging whether the rinsing solution provided by the present invention is feasible based on two aspects of sensitivity and sensitivity, the specific research materials are as follows:

[0077] (1) "Hepatitis C Virus Nucleic Acid Detection Kit (Fluorescence Quantitative PCR Method)" produced by Shanghai Haoyuan Biotechnology Co., Ltd. (Medical Device Registration Certificate Number: National Food and Drug Administration (Zhun) No. 2011 No. 3400554); This product contains nucleic ...

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Abstract

The invention discloses a rinsing liquid for nucleic acid extraction purification. The rinsing liquid comprises a buffer reagent, polyethylene glycol and a salt. The rinsing liquid reduces cross contamination in sample nucleic acid extraction purification, is free of an air drying process in extraction, reduces nucleic acid extraction purification time and improves nucleic acid extraction purification efficiency. A preparation method of the rinsing liquid has simple processes and can be operated easily.

Description

technical field [0001] The invention relates to a rinsing solution, in particular to a rinsing solution for nucleic acid extraction and purification. Background technique [0002] Nucleic acid is one of the most basic substances of life, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which widely exist in animal and plant cells, bacteria, viruses and other living organisms, and play a role in storing and transmitting genetic information . [0003] With the development of molecular diagnostic technology that uses nucleic acid as the detection object, nucleic acid detection has been widely used in clinical inspection, entry-exit inspection and quarantine, forensic evidence and other fields. One of the key links in nucleic acid detection is the extraction and purification of nucleic acids from various biological samples. The efficiency and purity of nucleic acid extraction play a crucial role in the success of downstream nucleic acid detection. [0004] The...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 何晓辉张健
Owner SUZHOU SYM BIO LIFESCI CO LTD
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