Detection method for microelements in peripheral blood

Abstract

The invention provides a detection method for microelements in peripheral blood. The detection method includes the following steps that 1, cleaning and disinfection are conducted; 2, a sample is acquired; 3, sample diluent is prepared; 4, detection liquid is prepared; 5, the concentration of the microelements is detected. A special acquisition tube is adopted for acquiring the sample in a scraping mode, the sample diluent is obtained by diluting an HNO3 and triton containing stock solution, and an inductively coupled plasma source mass spectrometer is applied to detecting the concentration of the microelements in the sample. The detection method for the microelements in the peripheral blood has the advantages that sample contamination is low, detection accuracy is high, the retention time of the sample is long, and the precision of the adopted instrument is high.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a method for detecting trace elements in peripheral blood, in particular to a method for detecting trace elements in peripheral blood with an inductively coupled plasma mass spectrometer. Background technique [0002] At present, the collection of peripheral blood in hospitals adopts the method of capillary siphon, and then puts the collected peripheral blood into the bullet containing diluent for detection, but the capillary contains more pollutants, which seriously affects the detection of trace elements results; and generally the peripheral blood is directly diluted at the sampling site and then sent for inspection, resulting in a very short storage time for the sample. In addition, when detecting trace elements in blood, inorganic salts, minerals and other substances contained in human blood will interfere with the detection and need to be completely removed, otherw...

AI Technical Summary

Problems solved by technology

[0002] At present, the collection of peripheral blood in hospitals adopts the method of capillary siphon, and then puts the collected peripheral blood into the bullet containing diluent for detection, but the capillary contains more pollutants, which seriously affects the detection of trace elements results; and generally the peripheral blood is directly diluted at the sampling site and then sent for inspection, resulting in a very short storage time for the sample

In addition, when detecting trace elements in blood, the inorganic salts, minerals and other substances contained in human blood will interfere with the detection and need to be completely removed, otherwise the test results will be inaccurate

[0003] At present, the detection of trace elements in peripheral blood is generally carried out by graphite furnace atomic absorption spectrometry and tungsten boat flameless atomic absorption spectrometry, but the above methods have the disadvantages of poor accuracy, low instrument precision, and complicated operation

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