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Detection method for microelements in peripheral blood

A technology of medium and trace elements and detection methods, which is applied in the field of medical detection, can solve the problems of affecting the detection results of trace elements, many pollutants in the capillary pipette, and inaccurate detection results, so as to improve the analysis speed and detection ability and high detection accuracy , the effect of prolonging the storage time

Inactive Publication Date: 2016-07-06
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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Problems solved by technology

[0002] At present, the collection of peripheral blood in hospitals adopts the method of capillary siphon, and then puts the collected peripheral blood into the bullet containing diluent for detection, but the capillary contains more pollutants, which seriously affects the detection of trace elements results; and generally the peripheral blood is directly diluted at the sampling site and then sent for inspection, resulting in a very short storage time for the sample
In addition, when detecting trace elements in blood, the inorganic salts, minerals and other substances contained in human blood will interfere with the detection and need to be completely removed, otherwise the test results will be inaccurate
[0003] At present, the detection of trace elements in peripheral blood is generally carried out by graphite furnace atomic absorption spectrometry and tungsten boat flameless atomic absorption spectrometry, but the above methods have the disadvantages of poor accuracy, low instrument precision, and complicated operation

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  • Detection method for microelements in peripheral blood

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Embodiment

[0031] A sort of Detection method of trace elements in peripheral blood , including the following steps:

[0032] (1) Cleaning and disinfection: Clean the blood collection site with pure water and disinfect it twice with 75% alcohol solution.

[0033] (2) Collect samples: After the above-mentioned blood collection site is naturally air-dried, puncture it with a blood collection needle, and then immediately turn the finger so that the cut of the finger faces down, so that the blood can flow out by itself, or gently squeeze with the finger far away from the puncture point Gently wipe off the first drop of blood with a clean sterile cotton swab, and then immediately use the peripheral blood collection tube to scrape it downwards and scrape it into the tube. The blood collection volume is required to be no less than 100 μL, roughly 3 drop; the inner wall of the above-mentioned collection tube is coated with heparin anticoagulant; the mouth of the collection tube is provided with ...

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Abstract

The invention provides a detection method for microelements in peripheral blood. The detection method includes the following steps that 1, cleaning and disinfection are conducted; 2, a sample is acquired; 3, sample diluent is prepared; 4, detection liquid is prepared; 5, the concentration of the microelements is detected. A special acquisition tube is adopted for acquiring the sample in a scraping mode, the sample diluent is obtained by diluting an HNO3 and triton containing stock solution, and an inductively coupled plasma source mass spectrometer is applied to detecting the concentration of the microelements in the sample. The detection method for the microelements in the peripheral blood has the advantages that sample contamination is low, detection accuracy is high, the retention time of the sample is long, and the precision of the adopted instrument is high.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a method for detecting trace elements in peripheral blood, in particular to a method for detecting trace elements in peripheral blood with an inductively coupled plasma mass spectrometer. Background technique [0002] At present, the collection of peripheral blood in hospitals adopts the method of capillary siphon, and then puts the collected peripheral blood into the bullet containing diluent for detection, but the capillary contains more pollutants, which seriously affects the detection of trace elements results; and generally the peripheral blood is directly diluted at the sampling site and then sent for inspection, resulting in a very short storage time for the sample. In addition, when detecting trace elements in blood, inorganic salts, minerals and other substances contained in human blood will interfere with the detection and need to be completely removed, otherw...

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Application Information

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IPC IPC(8): G01N27/62G01N1/10G01N1/38G01N27/626
CPCG01N1/10G01N1/38G01N27/62
Inventor 李淼谌贺宽子向玉珍唐春燕李慧源周梅华
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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