Applications of Chinese pine wood and extract thereof in medicine for treating osteoporosis
A technology for osteoporosis and osteoporosis, which is used in drug combinations, medical preparations containing active ingredients, medical raw materials derived from gymnosperms, etc.
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Embodiment 1
[0024] Example 1: Effect of Pinus tabulaeformis Extract on Acute Model of Rat Osteoporosis Induced by Retinoic Acid
[0025] SD female rats induced by retinoic acid were used to establish an acute model of rat osteoporosis. After administering 95% ethanol extract of Pinus tabulaeformis for a certain period of time, the second lumbar vertebrae were taken for paraffin section staining, and the bone density of the right femur was measured. The content of alkaline phosphatase and acid phosphatase in the serum was detected, and the changes of Pinus tabulaeformis on bone microstructure, bone mass and bone turnover of rats were observed.
[0026] 1. Experimental materials and methods:
[0027] 1.1 Main instruments and reagents: alkaline phosphatase detection kit (Nanjing Jiancheng Bioengineering Research Institute, A059-1), tartrate-resistant acid phosphatase detection kit (Nanjing Jiancheng Bioengineering Research Institute, A058), Spectra-Max multifunctional Microplate reader (Mol...
Embodiment 2
[0048] Example 2: 95% ethanol extract of Pinus tabulaeformis inhibits bone resorption by osteophagocytic cells
[0049] There are two types of cells in human bones, one of which is osteoclast, which specializes in destroying bones. After finding bones in the body, osteoclasts sit on the surface of the bone and begin to form a closed area. Once the closed period is formed, The calcium in the bone flows out, and the osteophagocytes begin to absorb the bone and form a cavity; another type of osteoblast continuously produces new bone and increases bone density. Generally speaking, before the age of 40, the functions of the two types of cells are in a state of balance and decline, but after the age of 40, the functions of the two types of cells will decline, and the decline rate of osteoblasts is much faster than that of osteophagocytes, so the bone mass begins to lose , resulting in a fragile bone structure, which is the so-called "osteoporosis".
[0050] Step 1: The osteophages ...
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