A bacterium and the chondroitinsulfate enzyme abc it produces

A technology of chondroitin sulfate enzyme and bacillus, applied in the direction of bacteria, microorganism-based methods, enzymes, etc., to achieve the effect of low cost, high product activity and good stability

Active Publication Date: 2019-10-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through a large amount of information retrieval, at the same time comparing the molecular weight of enzyme-producing microorganisms and enzymes, no chondroitinase ABC identical to the inventor's research was found.

Method used

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  • A bacterium and the chondroitinsulfate enzyme abc it produces

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Embodiment Construction

[0022] Below in conjunction with specific example the present invention will be further described

[0023] 1. A plant Acinetobacter sp .Screening and purification of C26 strain

[0024] Acinetobacter of the present invention Acinetobacter Acquisition of sp.C26 strain: collect intertidal mud from the east coast of Jiaodong, add it to 25mL enrichment medium (chondroitin sulfate 0.5g, yeast extract 5g, K 2 HPO 4 1g, MgSO 4 0.5g, NaCl15g, H 2 O 1000mL, pH7.2) Erlenmeyer flask, 28°C, 160rpm shake flask culture for 2 days, then dilute the enriched culture solution 100 times with sterile normal saline, take 0.1mL coating primary screening separation medium (chondroitin sulfate 0.5g, K 2 HPO 4 1g, MgSO 4 0.5g, yeast extract 5g, NaCl 15g, CaCl 2 0.01g, agar powder 18.0g, H 2 O 1000mL, natural pH) plates. Inverted at 28°C for 2 days, light yellow colonies with smooth edges were obtained on the selected plate, and a single colony was picked and cultured in the above isolati...

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Abstract

The invention belongs to the field of biotechnology and provides a bacterium and the chondroitinase ABC produced by it. The present invention relates to a kind of Acinetobacter ( Acinetobacter sp.C26), the strain has the characteristics of simple nutritional conditions, easy cultivation, and short generation time; the use of the strain of the present invention to produce chondroitin sulfate has the characteristics of high activity, good stability, and low cost, which can realize large-scale cultivation and meet the requirements of industrial applications.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an Acinetobacter and the chondroitinase ABC produced therefrom. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide, which is a kind of macromolecular polysaccharide formed by alternating connection of disaccharide units formed by glucuronic acid and hexosamine. It is widely distributed in animal and human cartilage, such as Animal throat bone, nasal bone, cartilage, sarcolemma and blood vessel wall, etc. There are many isomers of chondroitin sulfate. According to the different positions of the sulfate group in the hexosamine, it can be divided into chondroitin sulfate A (disaccharide unit: →4GlcAβ1, 3GalNAc4Sβ1→), chondroitin sulfate B ( Disaccharide unit: →4IdoAα1, 3GalNAc4Sβ1→), chondroitin sulfate C (disaccharide unit: →4GlcAβ1, 3GalNAc6Sβ1→), chondroitin sulfate D (disaccharide unit: →4GlcA2Sβ1, 3GalNAc6Sβ1→), etc. The latest research ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12R1/01
CPCC12N9/88C12Y402/0202C12Y402/02021C12N1/205C12R2001/01
Inventor 江晓路朱常亮
Owner OCEAN UNIV OF CHINA
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