Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A bacterium and the chondroitinsulfate enzyme abc it produces

A technology of chondroitin sulfate enzyme and bacillus, applied in the direction of bacteria, microorganism-based methods, enzymes, etc., to achieve the effect of low cost, high product activity and good stability

Active Publication Date: 2019-10-25
OCEAN UNIV OF CHINA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through a large amount of information retrieval, at the same time comparing the molecular weight of enzyme-producing microorganisms and enzymes, no chondroitinase ABC identical to the inventor's research was found.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A bacterium and the chondroitinsulfate enzyme abc it produces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Below in conjunction with specific example the present invention will be further described

[0023] 1. A plant Acinetobacter sp .Screening and purification of C26 strain

[0024] Acinetobacter of the present invention Acinetobacter Acquisition of sp.C26 strain: collect intertidal mud from the east coast of Jiaodong, add it to 25mL enrichment medium (chondroitin sulfate 0.5g, yeast extract 5g, K 2 HPO 4 1g, MgSO 4 0.5g, NaCl15g, H 2 O 1000mL, pH7.2) Erlenmeyer flask, 28°C, 160rpm shake flask culture for 2 days, then dilute the enriched culture solution 100 times with sterile normal saline, take 0.1mL coating primary screening separation medium (chondroitin sulfate 0.5g, K 2 HPO 4 1g, MgSO 4 0.5g, yeast extract 5g, NaCl 15g, CaCl 2 0.01g, agar powder 18.0g, H 2 O 1000mL, natural pH) plates. Inverted at 28°C for 2 days, light yellow colonies with smooth edges were obtained on the selected plate, and a single colony was picked and cultured in the above isolati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biotechnology and provides a bacterium and the chondroitinase ABC produced by it. The present invention relates to a kind of Acinetobacter ( Acinetobacter sp.C26), the strain has the characteristics of simple nutritional conditions, easy cultivation, and short generation time; the use of the strain of the present invention to produce chondroitin sulfate has the characteristics of high activity, good stability, and low cost, which can realize large-scale cultivation and meet the requirements of industrial applications.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an Acinetobacter and the chondroitinase ABC produced therefrom. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide, which is a kind of macromolecular polysaccharide formed by alternating connection of disaccharide units formed by glucuronic acid and hexosamine. It is widely distributed in animal and human cartilage, such as Animal throat bone, nasal bone, cartilage, sarcolemma and blood vessel wall, etc. There are many isomers of chondroitin sulfate. According to the different positions of the sulfate group in the hexosamine, it can be divided into chondroitin sulfate A (disaccharide unit: →4GlcAβ1, 3GalNAc4Sβ1→), chondroitin sulfate B ( Disaccharide unit: →4IdoAα1, 3GalNAc4Sβ1→), chondroitin sulfate C (disaccharide unit: →4GlcAβ1, 3GalNAc6Sβ1→), chondroitin sulfate D (disaccharide unit: →4GlcA2Sβ1, 3GalNAc6Sβ1→), etc. The latest research ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12R1/01
CPCC12N9/88C12Y402/0202C12Y402/02021C12N1/205C12R2001/01
Inventor 江晓路朱常亮
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com