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Composite material containing natural nano-hydroxyapatite and preparation method of composite material

A technology of hydroxyapatite and composite materials, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of high material density, easy accumulation, and low toughness of hydroxyapatite

Active Publication Date: 2016-08-03
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At the same time, due to the low toughness of a single hydroxyapatite material, dislocations will occur in the implanted body and the biodegradability is poor. The material has a high density and is easy to accumulate, which is not conducive to cell infiltration and growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the preparation of natural hydroxyapatite / carboxymethyl chitosan injectable composite material

[0056] (1) Fresh pig long bones that have just been slaughtered are collected from slaughterhouses under standardized management by special personnel, and contact with pollutants should be avoided as much as possible. After collection, they should be frozen and stored immediately.

[0057] (2) After the frozen material is thawed, it is fully cleaned, then cut into 5 cm long, and rinsed to remove surface attachments.

[0058] (3) Soak the sample in step (2) with 1g / 100ml TritonX-100 aqueous solution at 15±2°C for 10h, and then wash with water.

[0059] (4) Soak the sample in step (3) with 1 mol / L NaOH aqueous solution at 15±2°C for 1 hour, and then rinse with water.

[0060] (5) Soak the sample in step (4) with 1g / 100ml TritonX-100 aqueous solution at 15±2°C for 10h, and then wash with water.

[0061] (6) Soak the sample in the treatment step (5) with 3 mol / L ...

Embodiment 2

[0071] Embodiment 2, the preparation of natural hydroxyapatite / carboxymethyl chitosan sponge material

[0072] The injection-type composite material prepared in Example 1 was freeze-dried to obtain a natural hydroxyapatite / carboxymethyl chitosan sponge material, wherein carboxymethyl chitosan provided a tough support, and the natural hydroxyapatite was attached to the The surface of the scaffold acts as a bone guide.

[0073] The prepared sponge material was observed under a scanning electron microscope, as image 3 As shown, the prepared sponge material has a porous structure, which is conducive to cell growth and guides tissue regeneration; hydroxyapatite is evenly adsorbed on the surface of the scaffold to provide mechanical support and guide bone formation.

Embodiment 3

[0074] Embodiment 3, the preparation of natural hydroxyapatite / collagen protein sponge material

[0075] (1) Fresh long bones of pigs that have just been slaughtered are collected by special personnel from slaughterhouses under standardized management, and contact with pollutants should be avoided as much as possible, and frozen and stored immediately after collection;

[0076] (2) After the frozen material is thawed, it is fully cleaned, then cut into a length of 5 cm, and rinsed to remove surface attachments.

[0077] (3) Soak the sample in step (2) with 1g / 100ml TritonX-100 aqueous solution at 15±2°C for 10h, and then wash with water.

[0078] (4) Use 1mol / L NaOH solution aqueous solution at 45±2°C to soak the sample in step (3) for 1 hour, then rinse with water;

[0079] (5) Soak the sample in step (4) with 1g / 100ml TritonX-100 aqueous solution at 15±2°C for 10h, and then wash with water.

[0080] (6) Use 1% trypsin solution (the enzyme activity of trypsin is 300 units / m...

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Abstract

The invention discloses a composite material containing natural nano-hydroxyapatite and a preparation method of the composite material .The preparation method of the composite material comprises the following steps that an aqueous solution of a carrier is prepared to obtain an aqueous carrier solution, the aqueous carrier solution and the natural nano-hydroxyapatite are mixed and then subjected to irradiation sterilization, and the composite material is obtained, wherein collagen or carboxymethyl chitosan or hyaluronic acid is adopted as the carrier .The composite material prepared through the method mainly has two forms including the injection form and the sponge form; compared with the injection-form composite material, the sponge-form composite material has the main differences that freeze-drying is adopted, therefore, the preservation mode is simple, and use is convenient; the injection-form composite material has the advantage of being capable of being injected and used for filling of irregular defect sites.

Description

technical field [0001] The invention relates to a composite material containing natural hydroxyapatite and a preparation method thereof. Background technique [0002] Hydroxyapatite (Hydroxyapatite, referred to as HA) is the main inorganic component of human and animal bones and teeth, with a content of about 60 wt% in bone, and its basic unit is acicular apatite crystals. HA has good bioactivity and biocompatibility, and can form a tight combination with the soft and hard tissues of the human body in a short period of time after being implanted in the human body. It is a very good bone repair material. [0003] In animal hard tissue materials, HA exists in the form of composite materials. Human bone tissue is a geometric body formed by twisting and intertwining microcrystalline HA and collagen fibers in three-dimensional space. The formation of this composite material has undergone complex and lengthy biochemical reactions. Some data show that although human bone growth a...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/50
CPCA61L27/46A61L27/50A61L2430/02C08L5/08C08L89/00
Inventor 董佳桓闫玉芳王彬郭松孙先昌
Owner YANTAI ZHENGHAI BIO TECH
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