Method for preparing Chinese softshell turtle antihypertensive peptide by means of mixed enzymolysis and film filtration

A technology of mixed enzymatic hydrolysis and Chinese soft-shelled turtle, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of non-continuous operation, excessive degradation of substrates, low enzyme utilization rate, etc., and achieve easy industrialization Production, high conversion rate, good product quality

Inactive Publication Date: 2016-08-03
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the traditional enzymatic preparation of ACE inhibitory peptides has problems such as low enzyme utilization, excessive substrate degradation, and non-continuous operation. However, the mixed enzyme method-membrane filtration allows

Method used

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  • Method for preparing Chinese softshell turtle antihypertensive peptide by means of mixed enzymolysis and film filtration
  • Method for preparing Chinese softshell turtle antihypertensive peptide by means of mixed enzymolysis and film filtration
  • Method for preparing Chinese softshell turtle antihypertensive peptide by means of mixed enzymolysis and film filtration

Examples

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Effect test

Embodiment 1

[0030] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:

[0031] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, and then scald it with boiling water for 15 seconds to remove the Chinese soft-shelled turtle skin, remove the internal organs, bones, and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and after adding 5 times of water for homogenization, it is ready for use;

[0032] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to 4%, adjust the pH to 8 in a water bath at 50°C, add a mixed enzyme with 1% of the total mass of the substrate, and stir for enzymolysis After 2 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 39%, neutral protease 33%, trypsin 28%;

[0033] (3) Enzyme inactivation: at 95°C for 20 ...

Embodiment 2

[0038] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:

[0039] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, then scald it with boiling water for 20 seconds, remove the Chinese soft-shelled turtle skin, remove the internal organs, bones, and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and 10 times of water is added to homogenize it for later use;

[0040] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to 6%, adjust the pH to 9 in a water bath at 55°C, add a mixed enzyme with 2% of the total mass of the substrate, and stir for enzymolysis After 2 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 42%, neutral protease 30%, trypsin 28%;

[0041] (3) Enzyme inactivation: at 100°C for 15 minutes to inacti...

Embodiment 3

[0046] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:

[0047] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, then scald it with boiling water for 30 seconds, remove the Chinese soft-shelled turtle skin, remove the internal organs, bones and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and 3 times of water is added for homogenization and then set aside;

[0048] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to be 8%, in a water bath at 60°C, adjust the pH to 9.5, add a mixed enzyme with 3% of the total mass of the substrate, and stir for enzymolysis After 3 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 44%, neutral protease 32%, trypsin 24%;

[0049] (3) Enzyme inactivation: at 100°C for 15 minute...

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Abstract

The invention relates to the technical field of a preparation method for antihypertensive peptide, in particular to a method for preparing antihypertensive peptide through Chinese softshell turtles by means of mixed enzymolysis and film filtration. The method mainly comprises the key steps of raw material preprocessing, mixed enzyme hydrolysis, enzyme deactivation, centrifugal filtration, film filtration, freeze-drying and the like. Mixed enzyme in the mixed enzyme hydrolysis is prepared from, by mass, 39-44% of papain, 28-33% of neutral protease and 24-28% of trypsin. The mixed enzyme hydrolysis method is adopted for preparation, the enzyme utilization rate is high, the conversion rate and the inhibition rate of the Chinese softshell turtle antihypertensive peptide are both high, mixed enzyme hydrolysis and film filtration can be continuously conducted, continuous operation is achieved, industrial production is conducted easily, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of preparation methods of antihypertensive peptides, in particular to a method for preparing antihypertensive peptides from soft-shelled turtles by mixing enzymolysis and membrane filtration. Background technique [0002] Chinese soft-shelled turtle (Pelodiscus sinensis), commonly known as soft-shelled turtle and turtle, is mainly produced in China, Japan, Southeast Asia and other countries and regions. Chinese soft-shelled turtle aquaculture is an important part of my country's freshwater aquaculture industry, and it is also aquaculture with high economic benefits. type. Traditional Chinese medicine believes that soft-shelled turtle has the functions of nourishing yang and cooling blood, nourishing kidney and strengthening yang, clearing away heat and dissipating stagnation, nourishing qi and invigorating stomach, and the meat, blood, eggs, gallbladder, fat, nails and urine of Chinese soft-shelled turtle can...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 杨华张慧恩戚向阳陈世达
Owner ZHEJIANG WANLI UNIV
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