Detection method of bivalent manganese ions

A technology of divalent manganese ion and detection method, applied in the field of analytical chemistry, can solve the problems of low sensitivity, poor convenience, poor water solubility, etc., and achieve the effects of good water solubility, stable signal and low toxicity

Inactive Publication Date: 2016-08-10
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the fluorescent materials commonly used in fluorescent sensors are mainly organic fluorescent dyes and fluorescent quantum dots. However, the existing fluorescent materials have disadvantages such as high toxicity, low sensitivity, poor convenience and poor water solubility.

Method used

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  • Detection method of bivalent manganese ions
  • Detection method of bivalent manganese ions
  • Detection method of bivalent manganese ions

Examples

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Effect test

Embodiment 1

[0029] A method for synthesizing fluorescent copper nano-clusters, the synthesis method is as follows: first mix 9.4 mL of 12 mM thymine solution with 100 μL of 800 mM copper sulfate solution, stir for 5 min, then add 500 μL of 800 mM hydroxylamine hydrochloride solution, and stir for 1 min , adjusted the pH to 11.5 with 10M sodium hydroxide solution, and left to react for 2 hours to obtain fluorescent copper nanoclusters.

Embodiment 2

[0031] A detection method for divalent manganese ions, the method is: respectively adding different concentrations of Mn 2+ The solution is added to the fluorescent copper nanoclusters obtained in Example 1 diluted 1-fold, so that the Mn 2+ The concentrations are 0μM, 100μM, 120μM, 150μM, 180μM, 200μM, 250μM, 300μM, 350μM, 400μM, 450μM, and the fluorescence intensity values ​​are measured at the excitation wavelength of 354nm, and the results are shown in figure 1 , Figure 2a with Figure 2b .

[0032] Depend on figure 1 Obtain, the fluorescent copper nanocluster that embodiment 1 obtains can detect Mn sensitively 2+ , with Mn 2+ As the concentration increases, the fluorescence intensity of the fluorescent copper nanoclusters obtained in Example 1 increases gradually and the wavelength red shifts. When Mn 2+ At a concentration of 450 μM, the fluorescence of copper nanoclusters no longer increases.

[0033] Depend on Figure 2a with Figure 2b Gotta, Mn 2+ Relative...

Embodiment 3

[0035] Zn 2+ , Pb 2+ 、Cr 3+ 、Co 2+ 、Ni 2+ , Mg 2+ , Hg 2+ , Fe 3+ , Mn 2+ 、Cu 2+ , Ca 2+ Solution is added in the fluorescent copper nanocluster that the embodiment 1 of dilution 1 times obtains, makes each ion concentration be 200 μ M, measures its fluorescence intensity value respectively under 354nm excitation wavelength, calculates relative fluorescence intensity (F-F 0 ) / F 0 value, where: F 0 and F respectively represent the fluorescent intensity of the solution before and after the fluorescent copper nanoclusters obtained in Example 1 are added to the analyte, and the results are shown in image 3 .

[0036] Depend on image 3 Yes, yes Mn 2+ Response signal increases for other substances, especially for Mn 2+ The fluorescence is enhanced and the wavelength is red-shifted, which proves that the fluorescent copper nanoclusters obtained in Example 1 are paired with Mn 2+ Has high selectivity.

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Abstract

The invention relates to a method for detecting divalent manganese ions, which belongs to the technical field of analytical chemistry. The method comprises the following steps: ① first mix thymine solution and copper sulfate solution, then add hydroxylamine hydrochloride solution therein, and adjust the pH to 7 to 12, react for 1 to 2 hours, the molar ratio of thymine, copper sulfate and hydroxylamine hydrochloride is 2 to 5:2:2 to 40, to obtain copper nanocluster detection solution; ② will contain Mn 2+ The liquid to be tested is added to the product obtained in step ① to react for 1 to 10 minutes, and its fluorescence intensity value is measured at an excitation wavelength of 354nm. The beneficial effects of the present invention are: the detection method of the present invention has simple system, stable signal and good selectivity.

Description

technical field [0001] The invention relates to a method for detecting divalent manganese ions, belonging to the technical field of analytical chemistry. Background technique [0002] With the rapid development of modern industry, heavy metal pollution has become an important pollution problem. The main reasons include: sewage irrigation, mineral mining and smelting, waste gas and wastewater discharge from chemical plants, abuse of chemical fertilizers and pesticides, etc. The entry of heavy metal pollutants into lakes and reservoirs not only destroys the ecosystem, but also seriously threatens the safety of people's lives. [0003] As a heavy metal, manganese is one of the essential trace elements for the human body. Manganese deficiency symptoms can affect reproductive ability, and may cause congenital deformities in offspring, abnormal formation of bone and cartilage, and impaired glucose tolerance. In addition, the lack of manganese can cause neurasthenia syndrome and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 韩冰雁相荣超侯绪芬许杰于明波李莹彭婷婷
Owner DALIAN UNIV OF TECH
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