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Wolfberry anther culture breeding differential medium

A kind of differentiation medium and technology of Lycium barbarum flower, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of low efficiency of Lycium barbarum anther culture, to speed up the breeding process, improve efficiency, and reduce the degree of browning Effect

Active Publication Date: 2016-08-17
新沂时集创新创业科技产业园有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problem of low efficiency of culturing wolfberry anthers at present, and provides a kind of wolfberry flower which is specific for the stage of differentiation culture of wolfberry anther callus, has high differentiation rate of anther callus green shoots, good differentiation effect and light medium browning degree cultivar differentiation medium

Method used

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  • Wolfberry anther culture breeding differential medium
  • Wolfberry anther culture breeding differential medium
  • Wolfberry anther culture breeding differential medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Prepare the culture medium with the following formula:

[0036] Macroelements: KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙7H 2 O 1500mg / L, Glutamine 390mg / L, CaCl 2 ∙2H 2 O 220mg / L;

[0037] Trace elements: MnSO 4 ∙4H 2 O 18mg / L, ZnSO 4 ∙7H 2 O 27.5mg / L, H 3 BO 3 6.25mg / L, KI 0.8mg / L;

[0038] Iron salt and complexing agent: FeSO4 ∙7H 2 O 42.5mg / L, Na 2 -EDTA∙2H 2 O 56mg / L;

[0039] Organic ingredients: inositol 50mg / L, vitamin B1 0.4mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, adenine 22.5mg / L, proline 2.0mg / L, folic acid 0.4mg / L , Biotin 0.1mg / L;

[0040] Inorganic additives: activated carbon 9g / L, N-methyl-nitrosourea 27.5mg / L;

[0041] Plant growth regulator: 2,4-D 1.0mg / L, KT 1.7mg / L;

[0042] Physiologically active substances: Lycium barbarum root extract 12.5g / L;

[0043] Carbon source: sucrose 27.5g / L, fructose 13.5g / L;

[0044] Coagulant and other additives: plant gel 5.75g / L, sorbitol 22.5g / L, hydrolyzed protein 1.0g / ...

Embodiment 2

[0048] Prepare the medium with the following formula (optimized screening of carbon source combination):

[0049] Macroelements: KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙7H 2 O 1500mg / L, Glutamine 390mg / L, CaCl 2 ∙2H 2 O 220mg / L;

[0050] Trace elements: MnSO 4 ∙4H 2 O 18mg / L, ZnSO 4 ∙7H 2 O 27.5mg / L, H 3 BO 3 6.25mg / L, KI 0.8mg / L;

[0051] Iron salt and complexing agent: FeSO 4 ∙7H 2 O 42.5mg / L, Na 2 -EDTA∙2H 2 O 56mg / L;

[0052] Organic ingredients: inositol 50mg / L, vitamin B1 0.4mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, adenine 22.5mg / L, proline 2.0mg / L, folic acid 0.4mg / L , Biotin 0.1mg / L;

[0053] Inorganic additives: activated carbon 9g / L, N-methyl-nitrosourea 27.5mg / L;

[0054] Plant growth regulator: 2,4-D 1.0mg / L, KT 1.7mg / L;

[0055] Physiologically active substances: Lycium barbarum root extract 12.5g / L;

[0056] Coagulant and other additives: plant gel 5.75g / L, sorbitol 22.5g / L, hydrolyzed protein 1.0g / L.

[0057] Se...

Embodiment 3

[0060] Prepare the medium with the following formula (optimized screening of coagulants):

[0061] Macroelements: KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙7H 2 O 1500mg / L, Glutamine 390mg / L, CaCl 2 ∙2H 2 O 220mg / L;

[0062] Trace elements: MnSO 4 ∙4H 2 O 18mg / L, ZnSO 4 ∙7H 2 O 27.5mg / L, H 3 BO 3 6.25mg / L, KI 0.8mg / L;

[0063] Iron salt and complexing agent: FeSO 4 ∙7H 2 O 42.5mg / L, Na 2 -EDTA∙2H 2 O 56mg / L;

[0064] Organic ingredients: inositol 50mg / L, vitamin B1 0.4mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, adenine 22.5mg / L, proline 2.0mg / L, folic acid 0.4mg / L , Biotin 0.1mg / L;

[0065] Inorganic additives: activated carbon 9g / L, N-methyl-nitrosourea 27.5mg / L;

[0066] Plant growth regulator: 2,4-D 1.0mg / L, KT 1.7mg / L;

[0067] Physiologically active substances: Lycium barbarum root extract 12.5g / L;

[0068] Carbon source: sucrose 27.5g / L, fructose 13.5g / L;

[0069] Other additives: sorbitol 22.5g / L, hydrolyzed protein 1.0g / L. ...

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Abstract

The invention provides a wolfberry anther culture breeding differential medium and belongs to the technical field of tissue culture of wolfberry. The special medium is composed of major elements, minor elements, iron salt and complex agent, organic ingredients, inorganic additive, plant growth regulator, physiological activator, carbon source, coagulator and other additives. The wolfberry anther culture breeding differential medium is adopted to differentiate wolfberry anther callus, wolfberry anther callus green seedling differentiation rate is high, differentiation effect is good, and medium browning degree is low, so that wolfberry anther efficiency is improved greatly and breeding process is accelerated.

Description

technical field [0001] The invention relates to a differentiation medium for cultivating wolfberry flowers, in particular to a differentiation medium which is applied to the differentiation culture stage of wolfberry anther callus, has high green seedling differentiation rate, good differentiation effect and light medium browning degree, and belongs to wolfberry in the field of tissue culture technology. Background technique [0002] Lycium Barbarum (Lycium Barbarum. L), a plant of the genus Lycium in the Solanaceae family, is a perennial deciduous shrub. There are about 80 species of wolfberry in total, most of which are distributed in America, especially in South America. There are about 10 species in Eurasia, mainly distributed in Central Asia. There are 7 varieties and 3 varieties in my country, most of which are distributed in Northwest and North China. Wild species are distributed in Hebei, Inner Mongolia, Shanxi, Shaanxi, Gansu, Ningxia, Xinjiang, Qinghai and other ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张瑞明宋晓燕潭江
Owner 新沂时集创新创业科技产业园有限公司
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