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Preparation method of monodispersed fluorescent microsphere

A fluorescent microsphere and monodisperse technology, applied in the field of preparation of monodisperse fluorescent microspheres, can solve the problems of wide particle size distribution, stability to be improved, etc., and achieve the effects of uniform particle size, strong stability and high fluorescence efficiency.

Active Publication Date: 2016-08-17
SUZHOU KNOWLEDGE & BENEFIT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The fluorescent microspheres prepared by the prior art tend to have a wide particle size distribution, and the stability needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing monodisperse fluorescent microspheres, comprising the steps of:

[0028] (1) In an ice-water bath, with 1:1 volume ratio of acetone / dichloromethane mixed solution as solvent, dansyl chloride and allyl amine are used to synthesize dansyl chloride allyl amine fluorescent monomer;

[0029] (2) Add the dansyl chloride allyl amine fluorescent monomer into the polyethylene glycol / methanol mixed solution, stir evenly to form a fluorescent monomer suspension, and the dansyl chloride allyl amine fluorescent monomer accounts for the fluorescent monomer suspension 50% of the liquid weight; the dispersant weight is 8% of the dansyl chloride allylamine fluorescent monomer weight,

[0030] (3) Add styrene homopolymer seeds (particle size 0.50 μm, CV=3%) into the aqueous solution containing polyethylene glycol, stir well to form a uniformly dispersed seed suspension, and the styrene homopolymer seeds account for the seed suspension 50% of liquid weight; dispers...

Embodiment 2

[0035] A method for preparing monodisperse fluorescent microspheres, comprising the steps of:

[0036] (1) In an ice-water bath, a mixed solution of acetone and dichloromethane is used as a solvent to synthesize dansyl chloride allyl amine fluorescent monomers with dansyl chloride and allyl amine;

[0037] (2) Add the dansyl chloride allyl amine fluorescent monomer into the dextrin / ethanol mixed solution, stir evenly to form a fluorescent monomer suspension, the dansyl chloride allyl amine fluorescent monomer accounts for the weight of the fluorescent monomer suspension The weight of dextrin is 15% of the weight of dansyl chloride allylamine fluorescent monomer

[0038] (3) Add styrene-divinylbenzene copolymer seeds (particle size 55 μm, CV=3%) into the aqueous solution containing dextrin, stir well to form a uniformly dispersed seed suspension, and styrene-divinylbenzene copolymerization Seeds account for 45% of the weight of the seed suspension; the weight of dextrin is 8% ...

Embodiment 3

[0043] A method for preparing monodisperse fluorescent microspheres, comprising the steps of:

[0044] (1) In an ice-water bath, with acetone / dichloromethane mixed solution as solvent, dansyl chloride and allyl amine are used to synthesize dansyl chloride allyl amine fluorescent monomer;

[0045] (2) Add dansyl chloride allyl amine fluorescent monomer and acrylic acid monomer into polyethylene glycol / methanol mixed solution, stir evenly to form fluorescent monomer suspension, dansyl chloride allyl amine fluorescent monomer accounts for 40% of the weight of the fluorescent monomer suspension; the weight of the dispersant is 8% of the weight of the dansyl chloride allylamine fluorescent monomer,

[0046] (3) Add styrene homopolymer seeds (particle size 4.6 μm, CV=3%) into the aqueous solution containing polyethylene glycol, stir well to form a uniformly dispersed seed suspension, and the styrene homopolymer seeds account for the seed suspension 30% of liquid weight; dispersant ...

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Abstract

The invention discloses a preparation method of a monodispersed fluorescent microsphere. The preparation method comprises the following steps: 1, preparing a fluorescent monomer, and adding the fluorescent monomer to a dispersant / solvent to be stirred uniformly to obtain a fluorescent monomer suspension; adding polymer seeds to a dispersant containing aqueous solution to obtain a seed suspension; adding an initiator to the dispersant containing aqueous solution to obtain an initiator suspension; then adding the initiator suspension and the fluorescent monomer suspension to the seed suspension to react, and then placing a polymer microsphere suspension at the temperature of 25 to 40 DEG C to be subjected to vacuum mixing for 0.5 to 2 hours to obtain a monodispersed fluorescent microsphere suspension. The fluorescent microsphere prepared by the method has uniform particle size, controllable particle diameter, strong stability, high fluorescence efficiency, and potential application values in biological medicine fields of labeling, tracing, detection and the like.

Description

technical field [0001] The invention relates to a preparation method of polymer microspheres, in particular to a preparation method of monodisperse fluorescent microspheres. Background technique [0002] Fluorescent microspheres generally refer to microspheres marked with fluorescent substances on the surface of the microspheres or microspheres with fluorescent substances (such as embedding or aggregation) in the internal structure of the microspheres, which can excite fluorescence when stimulated by external energy. It is a functional microsphere loaded with fluorescent molecules, and its shape is mostly spherical. There are many methods for preparing fluorescent microspheres, which are generally divided into physical adsorption, self-assembly, embedding, chemical bonding, and copolymerization. The stability and reproducibility are not good due to the influence of medium and medium, but the properties of the fluorescent microspheres prepared by the copolymerization method ...

Claims

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Application Information

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IPC IPC(8): C08F257/02C08F226/02C08F220/06C08F220/32C08F220/60C08F216/14C09K11/02C09K11/06
CPCC09K11/02C09K11/06C08F257/02C09K2211/1011C09K2211/1007C08F216/1475C08F220/325C08F220/603
Inventor 阳承利李雪夏梦雨夏丽玲
Owner SUZHOU KNOWLEDGE & BENEFIT TECH CO LTD
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