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Method for obtaining eriodictyol by biologically transforming naringenin

A biotransformation and naringenin technology, applied in the biological field, can solve the problems of no hydroxylation, chemical structure and increased difficulty in chemically synthesizing flavonoids, and achieve the effects of easy separation, reduction of side reaction products, and simple operation procedures

Inactive Publication Date: 2016-08-24
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

In order to realize this process, chemical methods can be used. However, chemical synthesis is generally accompanied by the accumulation of toxic by-products, and does not have regio- or stereoselective hydroxylation of compounds. At the same time, the complex chemical structure increases the risk of chemical synthesis. The difficulty of flavonoids involves multi-step complex reactions and requires extreme reaction conditions such as high temperature and high pressure

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  • Method for obtaining eriodictyol by biologically transforming naringenin
  • Method for obtaining eriodictyol by biologically transforming naringenin
  • Method for obtaining eriodictyol by biologically transforming naringenin

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Embodiment Construction

[0020] Such as figure 1 As shown, the method for biotransforming naringenin of the present invention to obtain eriodictyol comprises the following steps:

[0021] 1. Obtain co-expression recombinant Escherichia coli

[0022] The sav7469 gene encoding CYP105D7 of Streptomyces avermitilis MA4680 (its gene sequence is shown in SEQID NO.1) is from CL_228_E11 cosmid (http: / / avermitilis.ls.kitasato-u.ac.jp) using primers by PCR method SEQ ID NO.2: 5'-GCCCATATGACAGAGCCCGGTACGTCCGTG-3' and reverse primer SEQ ID NO.3: 5'

[0023] - GCACTAGTTCAGCTTGCCGACCGCGGGAC-3' amplified. The camA gene and camB gene in the vector pT7NS-camAB (International Patent No. US 2006 / 0234337A1, whose gene sequence is shown in SEQ ID NO.4) respectively encode the pseudomonas redoxin reductase and pseudomonas of Pseudomonas putida cytoredoxin. The PCR amplification product and the vector pT7NS-camAB were digested with Nde I and Spe I, and ligated to obtain the recombinant vector pET11::sav7469::camA-camB (...

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Abstract

The invention discloses a method for obtaining eriodictyol by biologically transforming naringenin. The method comprises the following steps: preparing co-expressed recombinant bacillus coli at first; then carrying out preculture and enlarge culture on the recombinant bacillus coli; and after collecting thalli, biologically transforming the naringenin by using the thalli. The naringenin is transformed by a recombinant bacillus coli transformant, the eriodictyol as a single compound is generated in an orientated manner, hybridity of the product is reduced, meanwhile, steps of product separation and extraction are simplified, and difficulty in obtaining of the eriodictyol is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a method for obtaining eriodictyol by using recombinant Escherichia coli to biotransform naringenin. Background technique [0002] Flavonoids generally have a series of pharmaceutical properties: anti-anxiety, improvement of cardiac dysfunction caused by ischemia, and anti-estrogen effect on breast cancer cells. The basic chemical structure of these compounds consists of two benzene rings connected by a hybrid pyrone ring or pyran ring. Based on this structure, different subclasses can be obtained by substitution, namely isoflavones, flavones, catechols, and anthocyanins. Although the overall structure has great similarities among the subclasses and the different compounds within each subclass, their biochemical and biological properties can vary greatly with very slight modifications in the structure of flavonoids . The number and specific positions of the hydroxyl gro...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N15/53C12N15/70C12N1/21C12R1/19
CPCC12P17/06C12N9/0081
Inventor 许莲花刘玲姚秋萍
Owner ZHEJIANG UNIV
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