Detection method for content of lipid-soluble vitamins

The technology of a fat-soluble vitamin and a detection method is applied in the detection of fat-soluble vitamin content and the detection of fat-soluble vitamins in animal feed, which can solve the problems of large consumption of reagents, difficult to remove water, waste and the like, and achieve a simple pretreatment process. , the effect of reducing usage and improving efficiency

Active Publication Date: 2016-08-24
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) Repeated detection operations are cumbersome, time-consuming, and consume a lot of reagents;
[0008] (2) In the pretreatment, the traditional liquid-liquid extraction is carried out with ether, which is easy to emulsify, and it is necessary to use methods such as sodium chloride solution to demulsify, and then the demulsification effect of these methods is often unsatisfactory, and the extract needs to be extracted for a long time. Stand to layer
[0009] (3) The use of traditional liquid-liquid extraction technology requires the use of a lot of extraction solutions, resulting in a large waste of solvents;
[0010] (4) Ether is slightly soluble in water. After washing the ether extract, it is not easy to remove water. Anhydrous sodium sulfate needs to be used to remove water. Anhydrous sodium sulfate will absorb a certain amount of ether and cause vitamin loss;
[0011] (5) Transfer all the solid residues in the saponification reaction solution to the separatory funnel during extraction, which may easily cause the funnel to be blocked

Method used

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  • Detection method for content of lipid-soluble vitamins
  • Detection method for content of lipid-soluble vitamins
  • Detection method for content of lipid-soluble vitamins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The instrument and chromatographic conditions used in this embodiment are as follows:

[0041] 1. Instruments and equipment

[0042] Electronic balance (accurate to 0.1mg), laboratory routine glassware, ultrasonic extractor, 0.22μm microporous membrane, solvent filter, high performance liquid chromatography with UV detector, centrifuge, nitrogen blowing device.

[0043] 2. Chromatographic conditions

[0044] Chromatographic column: agilent Poroshell 120EC-C18 (2.7μm, 2.1mm×100mm)

[0045] Column temperature: 30°C

[0046] Mobile phase: (methanol: water) = (95:5)

[0047] Injection volume: 5μL

[0048] Program table for wavelength change

[0049] time (min)

Detection wavelength(nm)

0

324

3.2

264

5.3

285

[0050] A detection method for fat-soluble vitamin content, comprising the following steps:

[0051](1) Accurately weigh 1g of the feed to be tested, place it in a 250mL round bottom flask, add 50mL of 5g / L ascorbic a...

Embodiment 2

[0089] Using another brand of feed, using the same operation steps as in Example 1, the final results obtained are shown in Table 7.

[0090] Table 7 Detection results of fat-soluble vitamin content in the feed to be tested

[0091]

[0092] The present invention can effectively reduce the time of pretreatment operation, save the input of manpower and material resources, save the use of toxic reagents, and at the same time protect vitamins A and D 3 , E detection results are good, compared with the national standard method, the error is within 2.5%, there is no significant difference, it can accurately determine vitamin A, D in feed 3 , E content.

Embodiment 3

[0094] Using another brand of feed, using the same operation steps as in Example 1, the final results obtained are shown in Table 8.

[0095] Table 8 Detection results of fat-soluble vitamin content in the feed to be tested

[0096]

[0097] The present invention can effectively reduce the time of pretreatment operation, save the input of manpower and material resources, save the use of toxic reagents, and at the same time protect vitamins A and D 3 , E test results are good, compared with the national standard method, the error is within 2%, there is no significant difference, and it can accurately determine vitamin A and D in feed 3 , E content.

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Abstract

The invention relates to a detection method for the content of lipid-soluble vitamins. The detection method comprises the steps that a classical pretreatment mode of a saponification reaction is adopted, but traditional liquid-liquid extraction is replaced with ultrasonic extraction, and a to-be-detected solution and a standard solution are obtained through pretreatment; the standard solution and the to-be-detected solution are detected through liquid chromatography to obtain the retention time and the peak area of all components in a lipid-soluble vitamin standard substance and all components in to-be-detected feed, and a corresponding curve of the concentration and the peak area of the lipid-soluble vitamin standard substance is drawn; the retention time and the peak area of all the components of the to-be-detected feed are compared with a standard curve, and the content of the lipid-soluble vitamins in the to-be-detected feed is calculated. Compared with the prior art, the detection method has the advantages that the pretreatment process is simple, the usage amount of poisonous and harmful petroleum ether is decreased, and the method is more environmentally friendly and safer; in addition, a detection result is high in precision, the effect is good, and simultaneous separation and detection on the vitamin A, the vitamin D3 and the vitamin E are achieved in a short time.

Description

technical field [0001] The invention relates to the technical field of chemical component detection, in particular to a method for detecting the content of fat-soluble vitamins, in particular to a method for detecting fat-soluble vitamins in animal feed. Background technique [0002] Fat-soluble vitamins are vitamins that are insoluble in water but soluble in fat and organic solvents, including vitamin A, vitamin D 3 , vitamin E and vitamin K. Fat-soluble vitamins are essential nutrients for the growth and development of animals. In the natural environment, animals mainly obtain fat-soluble vitamins through food, but in artificial breeding, animals cannot meet their growth and development requirements simply by ingesting fat-soluble vitamins in food. and reproductive needs. Animals deficient in fat-soluble vitamins develop a range of diseases. For example, vitamin A deficiency can lead to problems such as decreased weight gain, movement disorders, blindness, reproductive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陈淋敏温庆琪吴有林张书金周盛昌肖丽萍姜文艾春香
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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