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Feed additive for promoting growth of porcine skeletal muscle and application thereof

A technology of feed additive and pig feed, applied in application, animal feed, additional food elements, etc., can solve the problems of carnosine proliferation and muscle growth that have not been seen, and can promote growth and development, improve growth performance and meat production efficiency , improve the effect of cell proliferation

Pending Publication Date: 2016-08-31
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the research on carnosine for pigs has mainly focused on its antioxidant properties and its effect on improving meat quality. There have been no reports of carnosine on pig skeletal muscle satellite cell Muscle growth, a report on the effect of carnosine on pig muscle growth at the cellular level

Method used

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  • Feed additive for promoting growth of porcine skeletal muscle and application thereof
  • Feed additive for promoting growth of porcine skeletal muscle and application thereof
  • Feed additive for promoting growth of porcine skeletal muscle and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Effects of Carnosine on the Proliferation of Porcine Skeletal Muscle Satellite Cells

[0017] Take five 96-well plates, and inoculate the third generation (F 3 ) Pig longissimus dorsi muscle satellite cells, when inoculated 24h, respectively process pig longissimus dorsi muscle satellite cells with carnosine of 0, 0.2, 2, 10, 20 and 40mM / L, each processing 10 repetitions (that is, 10 wells ). At 0, 24, 48, 72 and 96 hours after carnosine treatment, a 96-well plate was taken respectively, and the proliferation of satellite cells was measured by MTT method. The measured absorbance (OD) values ​​are shown in Table 1.

[0018] Table 1 The effect of carnosine on the proliferation of pig longissimus dorsi muscle satellite cells

[0019]

[0020] As can be seen from Table 1, the carnosine treatment of F with concentration of 0.2 and 2mM / L 3 Pig longissimus dorsi muscle satellite cells can significantly promote the proliferation of satellite cells; when the con...

Embodiment 2

[0021] Example 2: Effects of Carnosine on Pig Skeletal Muscle Satellite Cell Cycle

[0022] Take 12 6-well plates and inoculate the third generation (F 3 ) Pig longissimus dorsi muscle satellite cells were treated with 0, 0.2, 2 and 20 mM / L carnosine respectively 24 hours after inoculation, and 12 6-well plates were treated with 6 replicates (ie 6 wells) for each treatment. At 24, 48 and 72 hours after carnosine treatment, protein samples were collected, and the cell cycle of satellite cells was measured by flow cytometry. The results are shown in Table 2.

[0023] Table 2 The effect of carnosine on satellite cell cycle of pig longissimus dorsi muscle

[0024]

[0025]

[0026] As can be seen from Table 2, compared with the control group, with 2mM / L carnosine treatment F 3 Pig longissimus dorsi muscle satellite cells can significantly reduce the proportion of cells in the G0 / G1 phase after treatment for 24h and 48h, increase the proportion of cells in the S+M phase, th...

Embodiment 3

[0028] Example 3: Effects of carnosine on the mTOR signaling pathway of pig longissimus dorsi muscle satellite cells

[0029] The mTOR signaling pathway plays an important role in the synthesis of proteins. According to the results of Examples 1 and 2, 2mM / L carnosine was selected to treat satellite cells. After 48 hours of treatment, protein samples were collected, and proteins in the mTOR signaling pathway were measured by Western blot. The expression level, the measurement results are shown in Table 3 and figure 1 shown.

[0030] Table 3 Effects of carnosine on the mTOR signaling pathway in pig longissimus dorsi muscle satellite cells

[0031]

[0032]

[0033] from Table 3 and figure 1 It can be seen that carnosine significantly increased the expression levels of p-mTOR(2448) / mTOR and p-p70S6K / P70s6k in satellite cells of the longissimus dorsi muscle, and then activated mTOR and its downstream S6K, increased protein synthesis, and promoted Proliferation of satelli...

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Abstract

The invention discloses a feed additive for promoting growth of porcine skeletal muscle and application thereof in the technical fields of feed processing and fish breeding and poultry raising. The feed additive is a carnosine obtained from condensation of beta-alanine and L-histidine with a relative molecular weight of 226.24, and is a natural antioxidant. The feed additive is capable of activating an mTOR signal pathway and enhancing proliferation of porcine skeletal muscle satellite cells, thus promoting the growth of the porcine skeletal muscle. The mechanism of porcine muscle growth is explained in terms of cells and an important idea is offered for development of animal growth promoting additives. When the additive is added to a pig feed, the growth performance of pigs and the meat producing efficiency can be improved. The additive has remarkable economic benefits, is safe without any risk even in long-term use, and has the characteristics of going green, safety, and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of feed processing and aquaculture, and in particular relates to a feed additive for promoting the growth of pig skeletal muscle and its application. Background technique [0002] The total number of myofibers in porcine muscle is determined before birth, and muscle growth in later stages of the animal is only an increase in the diameter and length of the muscle fibers. The increase in the diameter and length of the muscle fiber in the later stage is related to the increase of its DNA capacity, but the muscle fiber does not have the ability to synthesize DNA. Skeletal muscle satellite cells provide a nucleus for the subsequent growth of muscle fibers by fusing with them and providing a source of DNA for muscle fibers. Therefore, an additive that can promote the proliferation of pig skeletal muscle satellite cells will play an important role in muscle growth. [0003] Carnosine is a natural water-soluble di...

Claims

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Application Information

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IPC IPC(8): A23K20/142A23K50/30
Inventor 马秋刚计成赵丽红刘涛
Owner CHINA AGRI UNIV
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