A nanosuspension for oral protein immunization and its preparation method

Abstract

The invention relates to a nano suspension for oral protein immunization and a preparation method thereof, and belongs to the field of medicine. Bovine serum albumin (BSA) is taken as the model drug, PLGA is taken as the carrier, and a solvent volatilization method is adopted to prepare a BSA loaded MCS / PLGA / BSA nano suspension. The particle size of the suspension is 532.8 nm, the zeta potential is 28.92 mv, and the encapsulation rate is 89.34%. Mannosylated chitosan (MCS) is adsorbed on the surface of PLGA / BSA nano particles through a static effect, thus the stability of nano particles in gastrointestinal liquid becomes more stable, moreover, the stay time in intestinal cavity is prolonged, and the nano particles with positive charges can be absorbed by cells more easily. Nano particles are transferred by epithelium related with follicle to PP nodes; through the combination between mannose ligands and mannose acceptors on antigen-presenting cells (APCs), the intake of APCs is increased, and thus the immunization reactions are induced.

Description

technical field

[0001] The invention relates to a nano-suspension for oral protein immunization and a preparation method thereof, belonging to the field of medicine. Background technique

[0002] Vaccines are one of the most effective ways to prevent infectious diseases. The mucosa is exposed to a large number of commensal and pathogenic microorganisms, and is the primary area for infection, and in a healthy adult, the mucosa-associated lymphoid tissue (MALT) accounts for 80% of all immune cells and is the largest mammalian lymphatic system. . However, the vast majority of marketed vaccines are intramuscular injection vaccines. Although intramuscular immunization can produce a strong systemic immune response, it cannot induce a strong immune response at the mucosal site. Therefore, compared with injectable vaccines, mucosal vaccines have many advantages, including inducing effective mucosal and systemic immune responses, producing mucosal IgA and serum IgG antibodies, avoi...