Method for preparing medical hemostatic sponge by resource utilization of notoginseng stems and leaves
A hemostatic sponge and resource-based technology, applied in the field of medical material preparation, can solve the problems of high infection rate, inability to improve material limitations, poor hemostatic efficiency, etc., and achieve the effect of simple preparation process
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example 1
[0018] First collect the mature notoginseng stems and leaves, wash them and dry them naturally, then grind and sieve to prepare 65 mesh notoginseng stems and leaves powder, then mix the notoginseng stems and leaves powder with deionized water at a mass ratio of 2:1 Stir and mix, and place at room temperature to ferment for 8 days; after the fermentation is completed, filter the fermentation broth and collect the filtrate, and then weigh 45 parts of deionized water, 30 parts of the above-mentioned filtrate, 8 parts of glucose, 5 copies of KH 2 PO 4 , 5 parts of MgS0 4 ·7H 2 0 and 7 parts of penicillin were placed in a petri dish, and cultured at 28°C for 40 hours to prepare a fermentation broth; in parts by weight, 35 parts of Panax notoginseng stem and leaf powder, 30 parts of the fermentation broth prepared above and Put 15 parts of sterile water in a fermenter, place the fermenter at 28°C, and culture it on a shaker at 200r / min for 5 days; Wash 3 times with deionized wat...
example 2
[0021] First collect the mature notoginseng stems and leaves, wash them and dry them naturally, then grind and sieve to prepare 72 mesh notoginseng stems and leaves powder, and then mix the notoginseng stems and leaves powder with deionized water at a mass ratio of 2:1 Stir and mix, and place at room temperature to ferment for 9 days; after the fermentation is completed, filter the fermentation broth and collect the filtrate, then weigh 50 parts of deionized water, 30 parts of the above-mentioned filtrate, 5 parts of glucose, 5 copies of KH 2 PO 4 , 5 parts of MgS0 4 ·7H 20 and 5 parts of penicillin were placed in a petri dish, and cultured at 29°C for 44 hours to prepare a fermentation broth; in parts by weight, 40 parts of Panax notoginseng stem and leaf powder, 30 parts of the fermentation broth prepared above and 30 parts of sterile water were placed in a fermenter, and the fermenter was placed at 29°C and cultured on a shaking table at 250r / min for 6 days; Wash 4 time...
example 3
[0024] First collect the mature notoginseng stems and leaves, wash them and dry them naturally, then grind and sieve to prepare 80-mesh notoginseng stems and leaves powder, and then mix the notoginseng stems and leaves powder with deionized water at a mass ratio of 2:1 Stir and mix, and place at room temperature to ferment for 10 days; after the fermentation is completed, filter it and collect the filtrate, then weigh 65 parts of deionized water, 25 parts of the above-mentioned filtrate, 3 parts of glucose, and 3 parts by weight, respectively. Part KH 2 PO 4 , 2 parts of MgS0 4 ·7H 2 0 and 2 parts of penicillin were placed in a petri dish, and cultured at 30°C for 48 hours to prepare a fermentation broth; in parts by weight, 55 parts of Panax notoginseng stem and leaf powder, 30 parts of the fermentation broth prepared above and 15 parts of sterile water were placed in a fermenter, and the fermenter was placed at 30°C and cultured on a shaking table at 300r / min for 7 days; ...
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