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Method for regenerating human hair keratin by means of L-cysteine

A cysteine ​​and keratin technology, applied in the field of waste human hair regeneration and utilization, can solve problems such as environmental impact and unfriendly reagents, and achieve the effects of sufficient resources, maximizing waste utilization and high molecular weight

Inactive Publication Date: 2016-09-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Human hair keratin molecules form a highly cross-linked three-dimensional stable structure through disulfide bonds, hydrogen bonds, salt bonds and other bonds. Due to the complexity and compactness of its structure, it does not dissolve under normal conditions. The most common method is to destroy the disulfide bonds or peptide bonds of protein molecules by strong bases, strong acids, reducing agents or oxidizing agents and decompose them into short peptides. These methods generally contain a large number of inorganic acids, alkalis, reducing agents, oxidizing agents and urea, which are not environmentally friendly. The discharge of reagents has a serious impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Send people to H 2 o 2 Wash in alkaline solution in which 30% H 2 o 2 The concentration of soda ash is 8mL / L, the concentration of soda ash is 10g / L, the temperature is 95°C, and the bath ratio is 40:1. Degrease with ethanol-acetone mixed solvent for 12 hours, and then crush the dried human hair; wherein, the cleaning temperature is 85°C, the bath ratio is 30:1, the degreasing agent used in the degreasing process can be soaked in human hair, ethanol- The volume ratio of ethanol to acetone in the acetone mixed solvent is 2:1.

[0030] (2) Add 5 g of crushed human hair into the L-cysteine-urea composite liquid, wherein the bath ratio is 20:1, the amount of L-cysteine ​​is 10 g / L, and the amount of urea is 8M. Use 200g / L NaOH to adjust the pH value to about 12, cover with a stopper, heat up to 75°C, and shake mechanically for 10 hours to obtain a mixed solution of human hair and keratin.

[0031] (3) Centrifuge the mixed solution of human hair and keratin at 9500 ...

Embodiment 2

[0037] (1) Send people to H 2 o 2 Wash in alkaline solution in which 30% H 2 o 2 The concentration of soda ash is 8mL / L, the concentration of soda ash is 10g / L, the temperature is 95°C, and the bath ratio is 40:1. Then degrease with ethanol-acetone mixed solvent for 12 hours, and then crush the dried human hair. Among them, the cleaning temperature is 85°C, the bath ratio is 30:1, the degreasing agent used in the degreasing process can be soaked in human hair, and the volume ratio of ethanol and acetone in the ethanol-acetone mixed solvent is 2:1.

[0038] (2) Add 5 g of crushed human hair into the L-cysteine-urea composite liquid, wherein the bath ratio is 20:1, the amount of L-cysteine ​​is 10 g / L, and the amount of urea is 2M. Adjust the pH value to about 12 with 200g / L NaOH, cover with a stopper, heat up to 85°C, and mechanically shake for 10 hours to obtain a mixed solution of human hair and keratin.

[0039] (3) Centrifuge the mixed solution of human hair and kerati...

Embodiment 3

[0045] (1) Send people to H 2 o 2 Wash in alkaline solution in which 30% H 2 o 2 The concentration of soda ash is 8mL / L, the concentration of soda ash is 10g / L, the temperature is 95°C, and the bath ratio is 40:1. Then degrease with ethanol-acetone mixed solvent for 12 hours, and then crush the dried human hair. Among them, the cleaning temperature is 85°C, the bath ratio is 30:1, the degreasing agent used in the degreasing process can be soaked in human hair, and the volume ratio of ethanol and acetone in the ethanol-acetone mixed solvent is 2:1.

[0046] (2) Add 5 g of crushed human hair into the L-cysteine-urea composite liquid, wherein the bath ratio is 20:1, the amount of L-cysteine ​​is 15 g / L, and the amount of urea is 8M. Adjust the pH value to about 12 with 200g / L NaOH, cover with a stopper, heat up to 85°C, and mechanically shake for 10 hours to obtain a mixed solution of human hair and keratin.

[0047] (3) Centrifuge the mixed solution of human hair and kerati...

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PUM

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Abstract

The invention relates to a method for regenerating human hair keratin by means of L-cysteine. The method comprises the steps of cleaning human hair fiber, then conducting degreasing, drying and smashing, adding obtained powder to L-cysteine-urea complex liquid with a bath ratio of (10:1)-(30:1), adjusting PH to be 10-12, conducting sealing, increasing temperature to 65-95 DEG C, conducting oscillation to obtain human hair and cysteine mixed solution, conducting centrifugal filtration, and taking filtrate as human hair keratin solution; conducting dialysis, adjusting PH to be 4.0-4.5, conducting standing after brown keratin is completely separated out, removing supernate, and conducting refrigeration and drying to obtain the human hair keratin. The method is simple and practical, and keratin powder high in molecular weight and extraction rate is prepared through dissolution and acetic acid precipitation of low-cost L-cysteine. The prepared keratin powder can be stored for a long time and can be applied to traumatology. The keratin powder can be dissolved in proper solvent for composite spinning with other polymers to be applied to bioengineering and other fields.

Description

technical field [0001] The invention belongs to the field of regeneration and utilization of discarded human hair, in particular to a method for regenerating human hair keratin by using L-cysteine. Background technique [0002] The use of discarded human hair has attracted the attention of many researchers since it was first used as a biological material in the 1980s. Because human hair grows on the human body, it has aroused great interest in the field of biological tissue engineering. Contains a large amount of melanin, which has a strong absorption of ultraviolet rays, and can also be used as an anti-ultraviolet functional additive. Human hair is similar to other animal hair such as wool, and its main component is α-keratin. The difference is that the diameter of human hair (about 80um) is significantly larger than that of animal hair (wool 20um). The reducing effect acts as a major hindrance and melanin is formed in the fibers. [0003] Human hair keratin molecules for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/06
CPCC08H1/06
Inventor 李戎朱惠惠吴兴乐
Owner DONGHUA UNIV