Method for producing small peptides through bionic enzymatic hydrolysis

A bionic enzymolysis and enzymolysis technology, applied in the biological field, can solve the problems of affecting the properties of small peptide products, large loss of product peptides, incomplete purification, etc., and achieve the effects of increasing use value, reducing losses, and high yield

Inactive Publication Date: 2016-09-07
安徽精准医疗产业研究院有限公司
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  • Application Information

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Problems solved by technology

[0006] In the existing process, due to incomplete refining and purification in the later stage, the loss of pro

Method used

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  • Method for producing small peptides through bionic enzymatic hydrolysis

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Embodiment 1

[0030] A method for producing small peptides by biomimetic enzymolysis, comprising the following steps:

[0031] (1) Grind ground beetle insects, pass through a 80-mesh sieve, add 10 times the weight of water to homogenate, add dilute hydrochloric acid, adjust the pH to 1.5-2, add pepsin with 1% raw material mass, and enzymolyze at 40°C for 1 hour ; Then, add sodium hydroxide solution to adjust the pH to 7.5-8, add trypsin with 1% raw material mass, and enzymolyze at 40° C. for 3 hours to obtain an enzymatic hydrolysis solution;

[0032] (2) Centrifuge the enzymatic solution at 4000-4500r / min for 10-15min, and take the supernatant. , take the supernatant, perform ultrafiltration with a hollow fiber membrane with a molecular weight cut-off of 3KD, and collect the permeate; after testing, the total peptide amount before filtration is 19.24g; after filtration, the total peptide amount is 19.02g, and the loss peptide amount is 1.1 %, almost no loss of total peptide amount.

[00...

Embodiment 2

[0040] A method for producing small peptides by biomimetic enzymolysis, comprising the following steps:

[0041] (1) Grinding donkey-hide gelatin, passing through a 80-mesh sieve, adding 15 times the weight of water to homogenate, adding dilute hydrochloric acid, adjusting the pH to 1.5-2, adding pepsin with a mass of 1.2% of the raw material, and enzymatically hydrolyzing at 37°C for 1.5h ; Then, add sodium hydroxide solution to adjust the pH to 7.5-8, add trypsin with 1% raw material mass, and enzymolyze at 37° C. for 4 hours to obtain an enzymatic hydrolysis solution;

[0042] (2) Centrifuge the enzymatic solution at 4000-4500r / min for 10-15min, and take the supernatant. , take the supernatant, perform ultrafiltration with a hollow fiber membrane with a molecular weight cut-off of 3KD, and collect the permeate; after testing, the total peptide amount before filtration is 19.24g; after filtration, the total peptide amount is 19.02g, and the loss peptide amount is 1.1 %, alm...

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Abstract

The invention discloses a method for producing small peptides through bionic enzymatic hydrolysis. The method is characterized in that the crushing and sieving mesh, the addition amount, the enzymatic hydrolysis temperature, the pH value and the enzyme addition amount are controlled and optimized; the addition amounts of pepsin and trypsin are small, so the cost is saved; and the yield of bionic enzymatic hydrolysis is high, and pepsin and trypsin combination is used to carry out bionic enzymatic hydrolysis in order to make the paste yield be 1.5-2 times that of traditional technologies. An ultrafilter membrane separation + electrodialysis + resin column chromatography combination technology is used to carry out separation and purification in the bionic enzymatic hydrolysis technology, the loss amount of active peptides is smaller than 2%, and the content of micro-molecular peptides with the relative molecular mass being smaller than 2KD is greater than 80%. The method has the advantages of reduction of the loss of the active peptides obtained through enzymatic hydrolysis, high content of the micro-molecular peptides, and improvement of use values.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for biomimetic enzymolysis to produce small peptides. Background technique [0002] The traditional nutrition view holds that protein nutrition is amino acid nutrition, and the need for protein is the need for essential amino acids and the synthesis of non-essential amino acids. That is to say, the protein that reaches the gastrointestinal tract must be hydrolyzed into free amino acids before it can be absorbed and utilized by the body. [0003] However, optimal performance was not obtained when animals were fed homozygous diets formulated with an ideal amino acid profile or low protein amino acid balanced diets. Therefore, some scholars put forward the view that animals have special needs for complete protein itself or for peptides, and the role of peptides in protein nutrition has also been paid more and more attention. [0004] Recent studies have shown that...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张斌张士伟陶少强李玉峰
Owner 安徽精准医疗产业研究院有限公司
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