Method for staining paraffin section of adult insect

Abstract

The invention provides a method for staining a paraffin section of adult insect, and belongs to the technical field of microscopic tissue section. The method includes tissue fixation, tissue dehydration, tissue transparency, waxdip, embedding and sectioning, section-flatting and section-drying, lost-wax and tissue rehydration, staining, dehydration and transparency, and section-sealing and observation. The final section has high distinction degree of the tissue, and the space structure of each organ and tissue of the insect body can be observed clearly. The method is used for the adult insect body material with hard body wall and low content of the body cavity liquid. The internal digestive system, excretory system, nervous system, respiratory system, circulatory system, and reproductive system can be observed clearly under the microscope by using the method to process the phorid adult insect body. The method for overall paraffin section of adult insect can fill the gap of observation of adult insect internal organ and tissue, can make up for the deficiency of partial observation, can provide classification feature of insect phylogenetic analysis, and has simple operation, short time consuming, and low cost.

Description

technical field [0001] The invention relates to a method for dyeing paraffin sections of adult insects, belonging to the technical field of microtissue sections, in particular to a method for dyeing paraffin sections of adult insects. Background technique [0002] Insect paraffin sectioning and staining techniques are important experimental techniques for studying insect tissue morphology and histopathology, and generally include steps such as dissection, fixation, dehydration and transparency, embedding, sectioning, and staining. At present, paraffin sectioning and staining techniques are mostly used for local tissues or organs of insect larvae and adults, such as antennae, digestive tract, and feet. In this way, the spatial structure of the overall internal organs and tissues of insect adults cannot be observed. The internal organ morphology of adults not only reflects their physiological characteristics, but also has more species stability because the internal structure ...

AI Technical Summary

Problems solved by technology

However, because the adult exoskeleton is hard and the body cavity contains less fluid, it is difficult to make a complete paraffin section by the conventional slicing method; the slicing method of the local tissue or organ of the adult is not only complicated to operate, but also takes a long time, and often requires advanced methods such as vacuum negative pressure treatment. Instruments and equipment, and more importantly, the effects of final fixation, embedding, and slicing are not ideal, and the final partial slicing has a very limited degree of tissue differentiation, making it difficult to observe the spatial structure of various systems and organs inside the complete worm body

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