Method for staining paraffin section of adult insect

A technology of paraffin sectioning and staining method, applied in sampling, measuring devices, instruments, etc., can solve problems such as difficulty in observing the spatial structure of systems and organs, limited tissue differentiation, complicated operation, etc., to make up for the lack of local observation, tissue The effect of high discrimination and simple operation

Inactive Publication Date: 2016-09-07
SHENYANG UNIV
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Problems solved by technology

However, because the adult exoskeleton is hard and the body cavity contains less fluid, it is difficult to make a complete paraffin section by the conventional slicing method; the slicing method of the local tissue or organ of the adult is not only complicated to operate, but also takes a long time, and often requires advanced methods such as va

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  • Method for staining paraffin section of adult insect
  • Method for staining paraffin section of adult insect

Examples

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Embodiment 1

[0030] In this embodiment, it is a method for paraffin sectioning of adult lice and flies, which includes the following steps:

[0031] 1. Tissue fixation

[0032] Prepare 0.5% TritonX-100-4% paraformaldehyde mixed fixative solution in a chemical fume hood.

[0033] The fixation process is as follows: collect the test donor worms and place them on an ice bed, remove the legs and wings, and put them directly into the mixed fixative solution. The ratio of the amount of fixative solution to the volume of the worms is 50:1. Hours; the test insects were adult psyllids raised in an artificial intelligence incubator at a temperature of 26°C and a relative humidity of 70%.

[0034] 2. Tissue dehydration

[0035] Take out the worms treated in (1), rinse with distilled water at 4°C, and then dehydrate with ethanol solutions with concentration gradients of 70%, 80%, 90%, 95%, and 100% at room temperature. The treatment time of each gradient is 20 minutes; the volume ratio of distilled...

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Abstract

The invention provides a method for staining a paraffin section of adult insect, and belongs to the technical field of microscopic tissue section. The method includes tissue fixation, tissue dehydration, tissue transparency, waxdip, embedding and sectioning, section-flatting and section-drying, lost-wax and tissue rehydration, staining, dehydration and transparency, and section-sealing and observation. The final section has high distinction degree of the tissue, and the space structure of each organ and tissue of the insect body can be observed clearly. The method is used for the adult insect body material with hard body wall and low content of the body cavity liquid. The internal digestive system, excretory system, nervous system, respiratory system, circulatory system, and reproductive system can be observed clearly under the microscope by using the method to process the phorid adult insect body. The method for overall paraffin section of adult insect can fill the gap of observation of adult insect internal organ and tissue, can make up for the deficiency of partial observation, can provide classification feature of insect phylogenetic analysis, and has simple operation, short time consuming, and low cost.

Description

technical field [0001] The invention relates to a method for dyeing paraffin sections of adult insects, belonging to the technical field of microtissue sections, in particular to a method for dyeing paraffin sections of adult insects. Background technique [0002] Insect paraffin sectioning and staining techniques are important experimental techniques for studying insect tissue morphology and histopathology, and generally include steps such as dissection, fixation, dehydration and transparency, embedding, sectioning, and staining. At present, paraffin sectioning and staining techniques are mostly used for local tissues or organs of insect larvae and adults, such as antennae, digestive tract, and feet. In this way, the spatial structure of the overall internal organs and tissues of insect adults cannot be observed. The internal organ morphology of adults not only reflects their physiological characteristics, but also has more species stability because the internal structure ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
CPCG01N1/2813G01N1/30G01N1/36
Inventor 刘广纯褚梦颖冯典兴
Owner SHENYANG UNIV
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