A kind of method for extracting and purifying anthocyanins in Aronia arvensis pomace

Abstract

The invention relates to a novel method for extracting and purifying anthocyanin in pomace of aronia melanocarpa. The novel method specifically comprises the following steps: superfine pulverizing, enzymolysis and extracting, low-speed centrifuging, high-speed centrifuging, ultrafiltration impurity removal, macroporous resin purification, nanofiltration, freeze drying and the like. After the pomace is pulverized by a vibratory ultrafine pulverizer until the average granularity of materials is greater than 300 meshes, pectinase and cellulolytic enzyme are added for enzymolysis. An obtained enzymolysis solution is successively centrifuged at low speed and high speed by a separating horizontal spiral centrifuge and a self-cleaning type disc separator successively. A settled solution is ultra-filtered by an ultrafiltration device. Ultra-filtered flowing-out liquid is adsorbed by macroporous resin chromatographic columns, and is eluted with 60%-90% ethanol to obtain the anthocyanin. An obtained anthocyanin ethanol solution is nano-filtered by a nanofiltration membrane, and ethanol as a solvent is removed. Trapped fluid of an anthocyanin solution is dried by a freeze vacuum drying method or a spray drying method until the water content of the finished product is smaller than or equal to 5%. Claret-colored aronia melanocarpa anthocyanin powder is obtained, and the content of the anthocyanin is 60%-90%.

Description

technical field [0001] The invention relates to a method for extracting and purifying anthocyanins from Sorbus arvensis pomace, and belongs to the technical field of agricultural product processing. Background technique [0002] Sorbus nigra belongs to Rosaceae and is a perennial deciduous shrub with purple-black berries. The plant is native to the northeastern United States, and now it is planted in Jilin, Heilongjiang, Liaoning, Inner Mongolia, Gansu, Jiangsu and other provinces and cities in China. This tree species has strong adaptability, rapid growth, beautiful tree shape, cold and drought resistance, and is suitable as an ornamental tree. In addition, its fruit is rich in various vitamins, amino acids, minerals, organic acids, terpenes, carotene and various nutritional and functional ingredients. It is worth mentioning that the fruit also contains more than 2.5% polyphenols, of which the content of anthocyanins is about 1%. In recent years, studies have shown that ...

AI Technical Summary

Problems solved by technology

So far, these methods generally have disadvantages such as environmental pollution, low extraction and purification efficiency and yield, cumbersome methods, and unrecoverable solvents, which are difficult to be applied in actual production.

In particular, impurities are not effectively removed during the purification process, resulting in low purity of anthocyanins, which seriously restricts the price of the product and affects its application in related fields