Reagent card box provided with pressing-type magnetic bead transfer mechanism

Abstract

The invention provides a reagent card box provided with a pressing-type magnetic bead transfer mechanism. The reagent card box comprises a first cavity, a second cavity, and a partitioning area for partitioning the first cavity and the second cavity, wherein an accommodating cavity is formed in the partitioning area, the interior of the accommodating cavity is provided with a pressing-type magnetic bead transfer mechanism used for transferring magnetic beads in the first cavity into the second cavity, the pressing-type magnetic bead transfer mechanism is embedded in the accommodating cavity in a sealed manner, and comprises a rotor and a push rod for pushing the rotor to rotate, and a cylinder-shaped rotation structure driven by the rotor is arranged on the bottom of the rotor. According to the scheme, the pressing-type magnetic bead transfer mechanism is arranged between the adjacent cavities, so that the magnetic beads and to-be-extracted materials adsorbed to the magnetic beads are sufficiently and efficiently transferred. In addition, the reagent card box is simple in structure and convenient to operate, and the complexity of a device matched with the reagent card box for use can be effectively reduced.

Description

technical field [0001] The invention relates to consumables used in the preparation and detection of nucleic acid or protein, in particular to a reagent cartridge provided with a push-type magnetic bead transfer mechanism. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) an...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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