A kind of enzyme-free superoxide anion electrochemical sensor and its preparation method and application

A superoxide anion, electrochemical technology, applied in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve the problems of limiting the development of enzyme sensors, cumbersome preparation of enzyme electrodes, difficult electron transfer, etc., to achieve ultra-sensitive electrochemical Responsive, not easy to store, easy to prepare effect

Active Publication Date: 2018-12-04
菏泽智慧新材料科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, electron transfer is difficult because the active center of the enzyme is covered by surface proteins
In addition, enzymes are expensive, easy to inactivate, have high environmental requirements, and the preparation of enzyme electrodes is cumbersome and difficult to store. These shortcomings greatly limit the development of enzyme sensors.

Method used

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  • A kind of enzyme-free superoxide anion electrochemical sensor and its preparation method and application
  • A kind of enzyme-free superoxide anion electrochemical sensor and its preparation method and application
  • A kind of enzyme-free superoxide anion electrochemical sensor and its preparation method and application

Examples

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Embodiment 1

[0036] A kind of preparation method of enzyme-free superoxide anion electrochemical sensor of the present invention is as follows:

[0037] a. Purification of multi-walled carbon nanotubes: Purification of multi-walled carbon nanotubes (MWCNTs) with mixed acid as oxidant, ultrasonication of the mixed solution of MWCNTs and concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1, and heating at 70 °C Reflux for 8 hours, wash with ultrapure water until neutral, and dry under vacuum at 50°C;

[0038] b. Preparation of L-cysteine-multi-walled carbon nanotube composites: take equal mass of purified multi-walled carbon nanotubes and L-cysteine ​​and disperse them in ultrapure water, then add 1-ethyl- (3-Dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide, both added in an amount 20 times the mass of carbon nanotubes (or L-cysteine) . 1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are used as cross-...

Embodiment 2

[0045] The difference between this example and Example 1 is that the concentration of silver nitrate is 0.01 mM, and the electrochemical deposition time is 300 s by chronoamperometry at 0 V. All the other steps are the same as in Example 1.

Embodiment 3

[0047] The differences between this example and Example 1 are: the concentration of silver nitrate is 3mM, and the electrochemical deposition time is 1s by chronoamperometry at 0V. All the other steps are the same as in Example 1.

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Abstract

The invention provides a production method of an enzyme-free superoxide anion electrochemical sensor. The production method comprises the following steps: functionalizing multi-walled carbon nano-tubes with L-cysteine to obtain Cys-MWCNTs, and depositing silver nano-particles on the surface of a Cys-MWCNTs modified electrode through an electrochemical technology to obtain the enzyme-free superoxide anion electrochemical sensor. The invention also provides the enzyme-free superoxide anion electrochemical sensor produced through the method, and an application of the enzyme-free superoxide anion electrochemical sensor. The catalysis effect of the silver nano-particles on active oxygen is used to substitute SOD enzyme for the first time in order to detect superoxide anions. The method has the advantages of simplicity, easiness in operation, difficult inactivation and realization of long-term preservation and use. The modified electrode is successfully applied to detection of the superoxide anions released by living cells (PC12). The modified electrode constructed in the invention has the characteristics of very good electrochemical response to O<2>.<->, wide linear range, high sensitivity, short response time, good stability and good repeatability.

Description

technical field [0001] The invention relates to an enzyme-free superoxide anion electrochemical sensor and its preparation method and application. Background technique [0002] Reactive oxygen species (ROS) are important intracellular signaling molecules that mainly regulate DNA damage, protein synthesis, apoptosis and so on. Among them, the superoxide anion (O 2 · − ), as one of the most important and active ROS, is involved in many physiological and pathological processes and can cause damage to biological organs. In recent years, O 2 · − The relationship between concentration and human health has attracted great attention. From the perspective of in vivo application, O 2 · − The dynamic detection linear range is wide, not only in the molar concentration of millimolar and micromolar, but even needs to be extended to the molar concentration of nanomolar level. At the same time, since O 2 · − It is extremely unstable and easily decays into other active oxygen units....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/333
CPCG01N27/333
Inventor 刘秀辉刘岳麟郭志盼刘一丹卢小泉
Owner 菏泽智慧新材料科技有限公司
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