Antimicrobial compositions for food packaging consisting of salicylaldehyde and carvacrol, thymol or their mixture
An antibacterial composition, a technology of thymol, applied in the field of compositions with antibacterial properties, can solve the problems of not showing broad spectrum and the like
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[0089] The fifth aspect also constitutes a part of the present invention. The fifth aspect is the preparation method of the active preparation of the second aspect of the present invention, which includes:
[0090] a1) providing a polymer matrix and optionally dissolving said polymer matrix in at least one solvent;
[0091] a2) optionally incorporating at least one plasticizer;
[0092] a3) optionally incorporating at least one anti-fog compound; and
[0093] a4) An antibacterial combination incorporating the first aspect of the invention.
[0094] In a particular embodiment of the fifth aspect of the invention, in step a2), a plasticizer is incorporated.
[0095] In a specific embodiment of the fifth aspect of the present invention, in a2), the at least one plasticizer is t-butyl citrate, polyadipate or glycerin.
[0096] In a specific embodiment, the active agent is an active coating for food packaging, and the solvent used in step a1) of the method is a solvent suitable ...
Embodiment 1
[0122] Example 1. Antimicrobial composition / combination. Synergy detection.
[0123] 1 – Materials and methods
[0124] 1.1 Reference microorganism
[0125] The antibacterial activity of solutions containing carvacrol, thymol and salicylaldehyde was tested against three different reference microorganisms:
[0126] - Gram-negative bacteria - Escherichia coli (strain ATCC 8739)
[0127] - Gram-negative bacteria - Pseudomonas aeruginosa (strain ATCC 27853)
[0128] - Gram-positive bacteria - Staphylococcus aureus (strain ATCC6538)
[0129] From the University of Valencia "Colección de Cultivos Tipo" (CECT). It was collected as a lyophilized culture and revived by regular inoculation on solid medium. After growth, the mother culture was kept frozen.
[0130] Microorganisms were grown an additional 3-4 times prior to analysis by diluting colonies in tryptic soy broth (TSB) and incubating overnight at 36°C.
[0131] 1.2 Preparation of inoculum
[0132] The day before the as...
Embodiment 2
[0169] Example 2. Coating Formulations.
[0170] Coating formulations were prepared as follows:
[0171] Carvacrol (C), thymol (T) and salicylaldehyde (SA) (ratio 3:1:8) were added to a polymer matrix (10% by weight) pre-dissolved in a food contact solvent. If necessary, add plasticizers and / or anti-fog additives. The amount of the mixture of active compounds is 10% by weight relative to the mass of polymer matrix in solution.
[0172] Polymer films containing carvacrol, thymol, and salicylaldehyde were prepared as follows:
[0173] The mixture was coated onto PP / PA polymer substrates at ambient temperature using a laboratory coater (ELCOMETER automatic film coater with sample temperature control and different transverse speeds). After coating, the film was dried at ambient temperature for 2 hours.
[0174] The obtained active films were comparable to conventional films in terms of transparency and gloss.
[0175] The amount of active compound present in the film after tr...
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