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A preparation method of bacterial cellulose membrane tissue engineering artificial tendon scaffold

A technology of bacterial cellulose membrane and artificial tendon is applied in the field of tissue engineering materials, which can solve the problems of high technical difficulty and achieve the effects of good mechanical properties and good cell compatibility.

Active Publication Date: 2019-09-10
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, tissue-engineered tendon has provided a more ideal and physiological method for clinical tendon defect repair. The main principle is to provide a cell growth scaffold that can meet the mechanical properties of tendon, and implant seed cells in vitro or in vivo. By regulating the formation of tendon-like tissue, the tendon defect can be repaired, but this method requires technical difficulty and the ideal scaffold material for tendon tissue engineering is very difficult to choose

Method used

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  • A preparation method of bacterial cellulose membrane tissue engineering artificial tendon scaffold
  • A preparation method of bacterial cellulose membrane tissue engineering artificial tendon scaffold
  • A preparation method of bacterial cellulose membrane tissue engineering artificial tendon scaffold

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Embodiment 1

[0041] 1. Preparation of BC membrane

[0042] (1) Preparation

[0043] 1. Gluconacetobacter xylosus ( Glucoacetobacter xylinus ) strains inoculated into the solid medium for routine activation, select the activated strains from the solid medium, put them into a 500ml Erlenmeyer flask containing 100ml of liquid medium, and place them on a shaker at 28°C and 160r / min Cultivate for 24-32 hours to make Gluconacetobacter xylosus seed solution for later use.

[0044]2. Gluconoacetobacter xylosus seed liquid is inoculated with 8% volume per liter of distiller's grain degradation liquid compound liquid, inserted into the distiller's grain degradation liquid compound liquid, fully shaken to make the bacteria liquid uniform, and then placed at 28°C Static culture in an incubator. After culturing for 5 days, it can be seen that a bacterial cellulose film floats on the surface of the liquid culture medium, and the thickness of the bacterial cellulose film is 3.1mm at this time.

[004...

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Abstract

The invention relates to a preparation method of a bacterial cellulose (BC) membrane tissue engineering artificial tendon scaffold. The technical scheme is as follows: the preparation method comprises the steps of inoculating a glucoacetobacter xylinus strain into a solid culture medium, activating and then culturing under the condition of a shaker for 24 to 32 hours to prepare a glucoacetobacter xylinus seed solution; inoculating the glucoacetobacter xylinus seed solution into a vinasse degradation liquid composite solution with the inoculum size of 6 to 8 volume percent, standing and culturing at 28DEG C for 4 to 6 days to obtain a bacterial cellulose membrane; taking the bacterial cellulose membrane, flushing, then soaking the bacterial cellulose membrane with distilled water for 24 to 28 hours to obtain a white semitransparent bacterial cellulose membrane. Compared with a traditional polymer material, the bacterial cellulose membrane tissue engineering artificial tendon scaffold disclosed by the invention has the beneficial effects that the bacterial cellulose membrane serves as a scaffold material, the porosity, the water binding capacity, the rehydration capability and mechanical properties meet the requirements; in addition, the bacterial cellulose membrane tissue engineering artificial tendon scaffold has the characteristics of nature, good cell compatibility and the like, and accords with the requirement of the tissue engineering artificial tendon scaffold.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials. Specifically, it relates to a method for preparing a bacterial cellulose membrane (BC membrane) tissue-engineered artificial tendon scaffold. Background technique [0002] Patients with tendon damage are often athletes and manual laborers. If the tendon is not repaired in time and cannot move, the patient feels physical and psychological pain. If treatment is given up, it will lead to disability. In recent years, tissue-engineered tendon has provided a more ideal and physiological method for clinical tendon defect repair. The main principle is to provide a cell growth scaffold that can meet the mechanical properties of tendon, and implant seed cells in vitro or in vivo. By regulating the formation of tendon-like tissue, the tendon defect can be repaired, but this method requires technical difficulty and it is very difficult to choose an ideal scaffold material for tendon tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/50C12P19/04C12R1/01
Inventor 黄镇黄祖新周文浩
Owner FUJIAN NORMAL UNIV
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