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Response surface method optimized subcritical water extraction method for ursolic acid in glossy privet leaves

A response surface method and subcritical water technology, applied in organic chemistry, steroids, chemical informatics, etc., to achieve good separation effect, optimized extraction process, and high economic value

Inactive Publication Date: 2016-09-28
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to extract the limiting factors of ursolic acid in privet leaves, provide a method for subcritical water extraction, and apply the response surface method to optimize the extraction and purification process of ursolic acid in privet leaves. The factors affecting the extraction rate of ursolic acid in privet leaves, such as liquid ratio, extraction time, extraction temperature and reaction pressure, are optimized, so as to improve the extraction rate of ursolic acid in privet leaves, reduce the extraction cost of ursolic acid, reduce environmental pollution, and save energy consume

Method used

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  • Response surface method optimized subcritical water extraction method for ursolic acid in glossy privet leaves
  • Response surface method optimized subcritical water extraction method for ursolic acid in glossy privet leaves
  • Response surface method optimized subcritical water extraction method for ursolic acid in glossy privet leaves

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Embodiment 1

[0032] Embodiment 1, a kind of extraction and purification method of ursolic acid in Ligustrum privet leaves optimized by response surface methodology:

[0033] 1. Determination of moisture content of privet leaf powder

[0034] Select and dry privet leaves, crush them, 80-200 meshes, put them into a sealed bag to avoid light, balance the water for 24 hours, weigh 2±0.1g privet leaf powder in a volumetric flask with constant weight, and place in an oven at 105±1°C Keep the weight constant for 4 hours, take it out and put it in a desiccator, weigh it after 30 minutes, and calculate the dryness.

[0035] 2. Extraction of ursolic acid from privet leaves by subcritical method

[0036] Weigh dry 1 g of privet leaf powder, mix evenly with deionized water at a certain solid-to-liquid ratio, place in a reaction kettle containing polytetrafluoroethylene, react at 120°C to 180°C, and cool down rapidly after the reaction is completed Bring the reaction solution to room temperature, cen...

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Abstract

The invention relates to a response surface method optimized subcritical water extraction method for ursolic acid in glossy privet leaves. The method comprises the following steps that a subcritical water method is used for extracting ursolic acid in the glossy privet leaves; a reaction still is cooled; solid-liquid separation is carried out; ethyl alcohol is added into sediment, shocking dissolution is carried out, and centrifugal separation is carried out; the content of ursolic acid in supernate is measured through high performance liquid chromatography (HPLC); through single factor analysis, the extraction process is optimized through a response surface optimizing method. Compared with the prior art, the method has the advantages of being simple and controllable in process, short in extraction time, clean, environmentally friendly, high in extraction rate, low in cost and the like, and is suitable for industrial production.

Description

technical field [0001] The invention relates to an extraction process of natural plant effective components, in particular to a response surface method to optimize the extraction and purification process of ursolic acid in privet leaves. Background technique [0002] Ligustrum lucidum Ait, also known as holly leaves and Oleaceae plants, is an evergreen tree with gray and smooth bark. It is widely distributed in the Yangtze River Basin and the south of the Yangtze River. It is recorded in "Compendium of Materia Medica": In addition to wind and blood, eliminating Swelling and pain relief, dizziness and pain in the head, various malignant sores, swollen sores, ulcers and festering, for a long time, boil it in water, apply it while hot, change it frequently, and boil it with rice vinegar. Aphtha of the mouth and tongue, swelling of the tongue, spitting out saliva with soaked juice. "Guizhou Folk Prescription Collection": External application stops bleeding due to injury, reduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00G06F19/00
CPCC07J63/008G16C99/00
Inventor 雷建都刘彦雪王璐莹刘静何静晋禄禄
Owner BEIJING FORESTRY UNIVERSITY
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