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Method for detecting levetiracetam in breast milk

A detection method and breast milk technology, which are applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem of no levetiracetam method, etc., and achieve the effects of fast and simple method, strong specificity and high sensitivity

Inactive Publication Date: 2016-10-05
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is no report on the detection method of levetiracetam in breast milk

Method used

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  • Method for detecting levetiracetam in breast milk
  • Method for detecting levetiracetam in breast milk
  • Method for detecting levetiracetam in breast milk

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0029] Embodiment 1 Quantitative detection method of the present invention

[0030] (1) Preparation of the test product: Take 180 μL of the test breast milk, place it in a 1.5 mL centrifuge tube, add 20 μL of acetaminophen standard solution with a concentration of 10 μg / mL, vortex and mix for 20 seconds, and use it as the internal standard test breast milk. Add 1 mL of ethyl acetate to the above-mentioned internal standard breast milk, vortex for 20 s, centrifuge at 4000 r / min for 10 min, absorb the upper layer of ethyl acetate into a 1.5 mL centrifuge tube, dry it with nitrogen in a water bath at 50 °C, add 100 μL of flow Vortex and mix for 20s after the phase, as the test product.

[0031] (2) Preparation of standard product: Take 160 μL of blank breast milk, place it in a 1.5 mL centrifuge tube, add 20 μL of acetaminophen standard solution with a concentration of 10 μg / mL, and then add 10, 20, 40, 100, 200 and 20 μL of 400 μg / mL levetiracetam standard solution, vortexed fo...

Embodiment 2

[0034] Embodiment 2 qualitative detection method of the present invention

[0035] (1) Preparation of the test product: Take 200 μL of the test breast milk, put it in a 1.5 mL centrifuge tube, add 1 mL of ethyl acetate, vortex for 20 s, centrifuge at 4000 r / min for 10 min, absorb the upper ethyl acetate layer into a 1.5 mL centrifuge tube Blow dry with nitrogen in a water bath at 50°C, add 100 μL of mobile phase and vortex for 20 seconds to serve as the test sample.

[0036] (2) Take the test sample for detection, the injection volume is 20 μL, and the chromatographic conditions are as follows: the chromatographic column is Diamonsil C18 (5 μm 150mm×4.6mm), and the mobile phase is methanol-potassium dihydrogen phosphate (0.01mol / L, pH4. 5) (17:83), isocratic elution; column temperature is 30°C; flow rate is 1mL / min; detection wavelength is 210nm.

[0037] In order to further illustrate the beneficial effect of the method of the present invention, the present invention provide...

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Abstract

The invention provides a method for detecting lamotrigine in breast milk. The method comprises the following steps: 1) preparation of a tested object: taking the test breast milk, adding ethyl acetate with five times of volume and uniformly mixing the materials, performing centrifugation, taking an ethyl acetate layer, drying the ethyl acetate layer, redissolving a mobile phase as the tested object; and 2)detection: taking the tested object in the step 1) and detecting by high performance liquid chromatography, wherein the detection condition is characterized in that a stationary phase is C18 chromatographic column; a mobile phase is a methanol-inorganic salting solution, the volume ratio of the stationary phase to the mobile phase is 17:83, the pH value of the inorganic salting liquid is 4.5, and the detection wavelength is 210 nm. The method provided by the invention can accurately detect levetiracetam in breast milk, has the advantages of rapid and simple process, strong specialization, high sensitivity and good stability, can be used for clinically detecting the concentration of levetiracetam, and provides guidance for woman with breast nursing who administrates levetiracetam.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical analysis, in particular to a method for detecting levetiracetam in breast milk. Background technique [0002] Levetiracetam (LEV, trade name KePPra) is a new generation of antiepileptic drugs, which belongs to pyrrolidone derivatives. Pyrrolidone nootropic drugs belong to the five-membered heterocyclic endamide nootropic drugs, which can be regarded as derivatives of γ-aminobutyric acid (GABA), and have special effects on receptors involved in neuroprotection and memory processes. A compelling class of nootropics with broad-spectrum, rapid-onset antiepileptic activity, favorable pharmacokinetic profile, good tolerability and safety profile, especially for refractory partial seizures in adults and children Adjuvant therapy for seizures. The structure and mechanism of action of levetiracetam are different from other antiepileptic drugs. Its pharmacokinetics has a linear relationship with the dosag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈蕾周东谢贞建
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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