Liquid-based thin cell preservation liquid and preparation method thereof

Abstract

The invention discloses a liquid-based thin cell preservation liquid and a preparation method thereof. The liquid-based thin cell preservation liquid is prepared from components as follows: 1%-3% of KCl, 1%-3% of KH2PO4, 0.1%-1% of Na2HPO4, 0.01%-0.1% of EDTA-2Na, 5%-10% of glycol, 70%-77.79% of dd H2O, 15%-20% of isopropanol and 0.1%-1% of formaldehyde. The liquid-based thin cell preservation liquid has the following advantages and effects: a KCl, KH2PO4 and Na2HPO4 mixed system is adopted, the concentration of Na<+> and K<+> in the liquid-based thin cell preservation liquid is regulated to keep reasonable osmotic pressure inside and outside cells when the cells are fixed, a neutral buffer system is formed, the morphology and the size of normal cervical exfoliated cells and diseased cervical exfoliated cells can be fixed, and the completeness and the reliability of preserved cells are improved; besides, moisture in the liquid-based cell preservation liquid is prevented from icing during low-temperature preservation of the cells due to adoption of a permeable anti-freezing agent prepared from glycol and isopropanol; finally, the sterilization capacity of the liquid-based thin cell preservation liquid is improved due to combined use of formaldehyde with lower concentration and EDTA-2Na while the cells are fixed, and the safety is improved.

Description

technical field [0001] The invention relates to the field of pathological detection of cervical cells, in particular to a liquid-based thin-layer cell preservation solution and a preparation method thereof. Background technique [0002] Liquid-based cell preservation solution is the main consumable for liquid-based cytology detection technology. Liquid-based thin-layer cytology examination system processing technology was born in 1991, and it was the first to be used in gynecological cytology examination. Since 2001, domestic research on liquid-based cytology screening for cervical cancer has made this technology develop rapidly. In gynecological cytology examination, liquid-based cell preservation solution is generally used to fix the sample cells in the solution, that is, to terminate any ongoing biochemical reactions, increase the mechanical strength and stability of the sample, and preserve the natural state of biological materials as close as possible. Save samples for...

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Problems solved by technology

[0004] However, this liquid-based cell preservation solution has the following disadvantages: after the chemical reaction between sodium phosphate buffer and calcium acetate and magnesium acetate, the neutral buffer system formed by magnesium phosphate calcium phosphate precipitation and sodium acetate, acetic acid and sodium phosphate buffer is destroyed. Acetic acid and sodium acetate formed after the chemical reaction form a new acidic buffer system. Acetic acid is corrosive, causing red blood cells in samples such as cervical mucus in cytopathological testing to rupture in an acidic and corrosive environment, and the ruptured red blood cells are released. The content, such as hemoglobin, will have adverse effects on subsequent analysis and detection, for example, the deposition of hemoglobin will hinder Megji staining, immunocytochemical detection, etc.

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