Method for producing Pingxiang cacai through rapid fermentation by virtue of compound bacteria
A compound bacteria and fast technology, applied in the field of food processing, can solve the problems of long pickling time of vegetables, complex metabolites of fermentation flora, and difficult control of miscellaneous bacteria, so as to shorten the fermentation time, improve the production efficiency and improve the inhibitory effect. Effect
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Embodiment 1
[0025] (1) Take 10 g of naturally fermented sauerkraut, cut it into pieces under a sterile environment, soak it in 90 mL of sterilized water, shake it for 30 min, and add sterile water to make a 10-fold dilution. Then take 1 volume of 10-fold dilution and add 9 volumes of sterilized water, mix well to make 10 -2 Diluent, and so on, to dilute the sample to 10 -7 times. choose 10 -3 Dilution, spread on the primary screening plate medium, and invert anaerobic culture at 36°C for 48 h, take the colonies with different shapes on the plate, separate them by streaking on the medium with the same composition as the primary screening medium, and repeat the separation for 3 After the second time, the strains of pure Lactobacillus plantarum, Lactobacillus buchneri, Issaccharomyces occidentalis and Issaccharomyces orientalis were screened out and stored on a slant at -4°C as the initial fermentation strains.
[0026] (2) Prepare MRS solid and liquid culture medium respectively, divide ...
Embodiment 2
[0032] (1) Prepare LBS liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 30 minutes, then set aside.
[0033] (2) Prepare PDA liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 30 minutes, then set aside.
[0034] (3) Purchase commercially available Lactobacillus plantarum, Lactobacillus brevis, Pichia pastoris, and Saccharomyces cerevisiae, respectively activate the strains according to the plate streaking method, inoculate them on the spare liquid medium in the plate, and culture at a constant temperature of 32°C for 3 days .
[0035] (4) The activated Lactobacillus plantarum and Lactobacillus brevis were transferred to LBS liquid medium, Pichia pastoris and Saccharomyces cerevisiae strains were transferred to PDA liquid medium, and cultured at 32°C for 3 days to obtain Lactobacillus plantarum , Lactobacillu...
Embodiment 3
[0038] (1) Prepare MRS liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, sterilize them by high-pressure steam at 121°C for 20 min, and set aside.
[0039](3) Prepare potato dextrose liquid culture medium, divide it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 20 minutes, then set aside.
[0040] (4) Purchase commercially available Lactobacillus pentosus, Enterococcus faecalis, Issa orientalis and Saccharomyces ruckeri, respectively activate the strains according to the plate streaking method, and inoculate them on the spare liquid medium in the plate respectively, and keep the temperature at 32°C Cultured for 3 days.
[0041] (5) The activated Lactobacillus pentosus and Enterococcus faecalis were transferred to the MRS liquid medium, and the strains of Issaccharomyces orientalis and Saccharomyces ruckeri were transferred to the potato dextrose liquid medium, and cultured at a constant...
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