Fluorescence silicon nanoparticle-based ratio type temperature sensor, and preparation method and application thereof

A silicon nanoparticle, temperature sensor technology, applied in chemical instruments and methods, thermometers with physical/chemical changes, thermometers, etc., can solve problems such as narrow suitable temperature range, harsh test conditions, and large cell damage, and achieve good response. low cost, good biocompatibility

Active Publication Date: 2016-10-12
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these temperature sensors have high sensitivity and accuracy, there are many problems, such as harsh test conditions, narrow suitable temperature range, greater damage to cells, poor applicability, etc.

Method used

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  • Fluorescence silicon nanoparticle-based ratio type temperature sensor, and preparation method and application thereof
  • Fluorescence silicon nanoparticle-based ratio type temperature sensor, and preparation method and application thereof
  • Fluorescence silicon nanoparticle-based ratio type temperature sensor, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of fluorescent silicon nanoparticles, the specific steps are as follows:

[0030] (1) Make a certain quality of trisodium citrate into an aqueous solution, and under the protection of nitrogen, inject APTES 8 to 9 times the mass of trisodium citrate into the above solution, react for 30 minutes, and then transfer the mixed solution to water Seal the heat reaction kettle and place it in an oven at 160°C for 5 hours;

[0031] (2) Dialyze the solution obtained in step (1) in deionized water for 4 times (change the water every 6 hours), and the molecular weight cut-off of the dialysis bag is 500Da, thereby preparing a blue fluorescent silicon nanoparticle aqueous solution, Adjust its concentration to 10mg / mL.

Embodiment 2

[0033] (1) Take 10mL of the aqueous solution of fluorescent silicon nanoparticles prepared in Example 1 in 3 flasks, numbered S1, S2, and S3, and then add 2.5 / 5.0 / 10.0mg of rhodamine B isothiocyanate in turn and fully Dissolve, and then use 0.1mol / L NaOH aqueous solution to adjust the pH of the mixed solution to weak alkaline (pH is about 8~9), and then stir and react in the dark for 48 hours.

[0034] (2) The solution after the reaction was dialyzed in water 4 times (change the water every 6 hours), and the molecular weight cut-off of the dialysis bag was 500Da, thus obtaining a ratiometric temperature sensor based on fluorescent silicon nanoparticles, with an average particle size of 2~ 3nm, such as figure 1 shown.

Embodiment 3

[0035] Example 3: Spectral properties of ratiometric temperature sensors

[0036] Get the S2 sample 100 μ L of embodiment 2 in the cuvette of 5 mL, dilute to 3 mL with deionized water, then test its ultraviolet-visible absorption spectrum, see figure 2 , there are two characteristic absorption peaks in the absorption spectrum, which are 350nm and 556nm respectively, which shows that the silicon nanoparticles and rhodamine B isothiocyanate are successfully combined together; then the fluorescence spectrum is measured with 360nm as the excitation wavelength, See image 3 , the test results show that the temperature sensor sample has fluorescence emission peaks at 445nm and 576nm.

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Abstract

The invention discloses a fluorescence silicon nanoparticle-based ratio type temperature sensor and an application thereof. When the temperature is 20-60DEG C, fluorescence of fluorescence silicon nanoparticles is insensitive to the temperature, fluorescence of rhodamine B isothiocyanate has good response to the temperature, and fluorescence emission peaks of the fluorescence silicon nanoparticles and the rhodamine B isothiocyanate do not interfere each other, so the rhodamine B isothiocyanate can be combined with the fluorescence silicon nanoparticles through covalent bonds to synthesize the novel fluorescence ratio type temperature sensor. Compared with present fluorescence temperature sensors, the ratio type temperature sensor disclosed in the invention has the advantages of good water solubility, simplicity in preparation, low cost, good biocompatibility, simple and fast detection method, high sensitivity and accuracy, and strong interference resistance. The sensor disclosed in the invention is suitable for practical production application and promotion, and has wide application prospects in the fields of intracellular temperature monitoring, cell imaging and environment monitoring.

Description

technical field [0001] The invention relates to the technical field of material preparation and analysis and detection, and mainly provides a ratiometric temperature sensor based on fluorescent silicon nanoparticles, a preparation method and an application. Background technique [0002] As we all know, temperature is an important physical parameter. In biological systems, temperature controls various physiological reactions of cells and is closely related to various physiological activities in complex cellular environments, such as cell structure and function, various enzyme reactions, cell division, gene expression, and metabolism. This is mainly because temperature can affect the equilibrium constants and reaction kinetics of various reactions in cells. For example, due to differences in intracellular metabolism, the temperature of malignant tumor cell tissues is higher than that of normal cells. Temperature not only has a huge impact on biological systems, but also has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/02G01K11/00
CPCC09K11/025C09K11/06G01K11/00
Inventor 陈建吕锟张培盛钟维邦王宏洪永想余茂林
Owner HUNAN UNIV OF SCI & TECH
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