A kind of lactobacillus plantarum and its culture and isolation method, screening method and application
A technology of Lactobacillus plantarum and culture, applied in the field of microorganisms, can solve problems such as large workload, low efficiency, complicated operation, etc., and achieve the effects of wide temperature adaptability, strong acid-producing ability and fast growth rate
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Embodiment 1
[0049] Embodiment 1: A kind of Lactobacillus plantarum, said Lactobacillus plantarum is classified as: Lactobacillus plantarum, preserved in China Center for Type Culture Collection CCTCC, the preservation number is CCTCC M 2016037, and the preservation date is January 14, 2016.
[0050] A bacteriocin, the bacteriocin is prepared by fermentation of the above-mentioned Lactobacillus plantarum.
[0051] An application of the above-mentioned Lactobacillus plantarum, wherein the Lactobacillus plantarum is used for fermenting and preparing fruit and vegetable vinegar, grain vinegar and fruit and vegetable enzymes.
[0052] The morphological characteristics of the Lactobacillus plantarum bacterial strain of embodiment one:
[0053] Description of colony morphological characteristics: such as figure 1 As shown, the colony is round, the diameter of the colony is 0.6 ~ 1mm, the colony is raised into a nearly spherical shape, the surface is smooth, and milky white. It has a sour, numb...
Embodiment 2
[0055] Embodiment 2: a kind of method for cultivating and separating the above-mentioned Lactobacillus plantarum, comprising the following steps: taking the fermented mash samples in the middle and late stages of the natural fermentation of mixed fruit and vegetable enzymes, drawing after dilution and applying to the separation medium plate, and culturing at 25°C for 2 On the second day, single colonies with a colony size of 0.5-1.1mm, a round shape, and a milky white color were picked, transferred to a medium slant, and cultured at 38°C for 2 days to obtain Lactobacillus plantarum.
[0056] Preferably, the separation medium on the separation medium plate is conventional medium, oligonutrient medium and simulated environment medium,
[0057] The conventional medium is tomato agar medium, tomato juice agar medium III, BCP medium, beef extract peptone medium, MC medium, MRS medium and LB medium;
[0058] The preparation method of the oligonutrient medium comprises the following ...
Embodiment 3
[0081] Embodiment three: a kind of cultivation and separation method of above-mentioned Lactobacillus plantarum, comprising the following steps: take the fermented mash samples in the middle and later stages of natural fermentation of mixed fruit and vegetable enzymes, absorb and apply to the separation medium plate after dilution, and culture at 25°C for 2 On the second day, single colonies with a colony size of 0.5-1.1mm, a round shape, and a milky white color were picked, transferred to a medium slant, and cultured at 38°C for 2 days to obtain Lactobacillus plantarum.
[0082] Preferably, the separation medium on the separation medium plate is one of conventional medium, oligonutrient medium and simulated environment medium,
[0083] Described conventional medium is the one in tomato agar medium, tomato juice agar medium III, BCP medium and beef extract peptone medium;
[0084] The preparation method of the oligonutrient medium comprises the following steps: respectively weig...
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