Method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen

A technology of kiwi fruit and pollen, which is applied in the fields of botanical equipment and methods, biocides, chemicals for biological control, etc., to achieve the effects of reducing pesticide residues, simple operation, and reducing secondary pollution

Active Publication Date: 2016-10-26
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the prevention and treatment of kiwifruit canker mainly focuses on the screening of c

Method used

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  • Method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen
  • Method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen
  • Method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example one

[0038] The bactericidal effect of chlorine dioxide aqueous solution, Thiamthenone, and Kelvin 3000 on Psa

[0039] (1) Psa culture

[0040] (2) Three bactericidal agents and their concentrations

[0041] ClO 2 The concentration of the solution is 20mg / L, 10mg / L, 1mg / L; the thiophene ketone is 500μL / L, 250μL / L, 100μL / L; the three thousand solution concentrations are 50mg / L, 25mg / L, 10mg / L L.

[0042] (3) The bactericidal effect of three medicaments on Psa

[0043] Test group: Use a pipette to pipette 1 mL of the prepared bacterial suspension into a 10 mL centrifuge tube, and then add 4 mL of the above three medicaments with different concentrations into the centrifuge tube, and mix thoroughly. Set the time gradient of each solution to the bacterial suspension as 15min, 30min, 45min, 60min, after the action (ClO 2 After the treatment group acts, take 0.5 mL of the sample solution and add 0.5 mL of neutralizer, neutralize for 10 minutes, and pay attention to the conve...

Example Embodiment

[0049] Example two

[0050] Study on the Influence of Three Kinds of Medicaments on Pollen Viability

[0051] (1) Concentration and processing time of the three agents

[0052] 10mg / L ClO 2 Solution treatment for 45min; 500μL / L thiamycin solution for 30min; 10mg / L can kill 3000 solution for 15min.

[0053] (2) Three medicaments to treat pollen

[0054] Test group: Use a pipette to draw 1 mL of pollen suspension into a 10 mL centrifuge tube, and then add 1 mL of the above three agents with different concentrations into the centrifuge tube, and mix well. Set the action time gradient to 15min, 30min, 45min, (ClO 2 After the treatment group acts, take 0.5 mL of the sample solution and add 0.5 mL of neutralizer, neutralize for 10 minutes, and pay attention to the conversion of the dilution factor in the final count)

[0055] Control group: Use a pipette to suck 1ml pollen suspension into a 10ml centrifuge tube, add 1mL sterile water to the centrifuge tube, and mix well. Let stand for 30min ...

Example Embodiment

[0062] Example three

[0063] ClO 2 Effect of Solution Treatment of Pollen on the Yield and Storage Quality of Kiwifruit

[0064] (1) Concentration and processing time

[0065] ClO 2 The solution concentration is 10mg / L, the treatment time is 45min, and the neutralizer is added immediately after the set time is reached.

[0066] (2) Pollination

[0067] Choose 4 ‘Hayward’ kiwi fruit trees that are healthy, disease-free and similar in vigor in the kiwi plantation. 2 Solution-treated pollen is used for pollination, and the pollination is carried out at the full blooming stage. The pollination is carried out once when the flower reaches 60%, 85%, and 100%, and then bagging immediately after pollination.

[0068] (Control group) Fruit tree No. 1-4, spray pollen solution was prepared normally, and after configuration, it was placed for half an hour and then sprayed with a sprayer.

[0069] (3) Fruit set rate survey

[0070] One month after pollination, the fruit-setting rate was calculated.

[0...

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Abstract

The invention provides a method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen. By adoption of ClO2 aqueous solution for treating the kiwifruit pollen, Psa in the pollen can be killed effectively while pollen activity is less affected; by killing of the Psa in the kiwifruit pollen, Psa propagation can be effectively controlled to reduce canker, and sustainable development of the kiwifruit industry is guaranteed. The ClO2 aqueous solution treatment method is combined with pollen suspension prepared before liquid pollination, and the ClO2 aqueous solution in an appropriate amount is added into the pollen suspension to kill Psa. Therefore, simplicity in operation, freeness of complex equipment and low cost are realized.

Description

technical field [0001] The invention relates to the technical field of fruit and vegetable disease prevention and control, in particular to a method for killing Psa in kiwi pollen. Background technique [0002] In recent years, the large-scale outbreak of kiwifruit bacterial canker has led to a decline in kiwifruit production, seriously affecting the development of the industry. Kiwifruit canker is a bacterial disease caused by Pseudomonas syingae pv.actinidiae (Psa). With the help of natural conditions such as insects and wind and rain, the pathogens independently infect the shoots of kiwifruit plants, leaf marks of branches, new forks, lenticels of leaves, stomata, etc., or the above tissues with poor nutrition, and then spread into each branch of the fruit tree to cause The whole plant is infected; or spread through pruning wounds and frost cracked wounds. [0003] Gallelli.A et al. (2011) found that canker pathogens existed in kiwifruit pollen in Italy through molecula...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01N59/00A01P1/00
CPCA01H1/02A01N59/00
Inventor 高贵田曹凡朱丹杜莹琳
Owner SHAANXI NORMAL UNIV
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