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Antibiotic-free feed for resisting porcine diarrhea

A technology for non-antibiotic feed and pig diarrhea, applied to livestock non-antibiotic feed and its application field, to achieve the effects of reasonable compatibility, improvement of pig diarrhea, and significant curative effect

Active Publication Date: 2016-10-26
浙江伊杰斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] At present, there is no report on the application of the combination of Trichoderma longhandle, Aspergillus niger and Candida tropicalis as an antibiotic-free feed additive for pigs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1, long handle trichoderma liquid

[0034] Inoculate Trichoderma longifolia (strain number ACCC30150) into PDA liquid medium, and culture at a constant temperature of 37° C. for 48 hours to obtain Trichoderma longi handle liquid.

[0035] The PDA liquid culture medium is: 200 grams of potatoes, 20 grams of glucose, and 1000 milliliters of sterile water.

Embodiment 2

[0036] Embodiment 2, the preparation of aspergillus niger liquid

[0037] Aspergillus niger (strain number CICC40613) was inoculated onto Czapek's agar medium, cultured at 28°C for 72h, then inoculated into Czapek's liquid medium, and incubated at 28°C for 72h to obtain Aspergillus niger liquid.

[0038] The Czapek's agar medium is: 3% sucrose, 0.3% sodium nitrate, 0.05% magnesium sulfate heptahydrate, 0.05% potassium chloride, 0.001% ferrous sulfate tetrahydrate, 0.1% potassium hydrogen phosphate, 1.5% agar, pH Value 6.0-6.5. Czapek's liquid medium without adding agar.

Embodiment 3

[0039] Embodiment 3, the preparation of Candida tropicalis liquid

[0040] Candida tropicalis (strain number ACCC20005) was inoculated on wort agar medium, cultured at 28° C. for 72 hours, then inoculated into wort liquid medium, and cultured at 28° C. for 72 hours to obtain Candida tropicalis liquid.

[0041] The wort juice agar medium is as follows: the wort juice is 5-6 degrees Baume, the pH value is 6.5, and 2g / 100ml of agar is added. Wort juice liquid medium without adding agar.

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PUM

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Abstract

The invention relates to an antibiotic-free feed which is prepared by the following steps: adding compound fungal liquidinto an assorted material, and carrying out solid fermentation. The compound fungal liquid is prepared by mixing the following fungal liquids in parts by weight: 1-3 parts of trichoderma longibrachiatum fungal liquid, 1-3 parts of aspergillus niger fungal liquid and 1-3 parts of candida tropicalis fungal liquid. Compared with common pig feeds, the invention has the following advantages: the antibiotic-free feed is rationally compounded, obvious in curative effects, and capable of obviously reducing the diarrhea rates of the pigs; tests have proven that the antibiotic-free feed has specific effects of improving the porcine diarrhea; and the feed is free of western medicine antibiotic ingredients, so that the feed disclosed by the invention is free of toxic and side effects, free of drug resistance, and free of pollution.

Description

technical field [0001] The invention relates to the field of feed, in particular to an antibiotic-free feed for livestock and its application. Background technique [0002] Since the middle of the 20th century, adding antibiotics to feed has played a positive role in preventing animal diseases, promoting animal growth, increasing feed remuneration, and increasing the output of livestock and poultry products. With the widespread application of antibiotics as feed additives, its various disadvantages have also been recognized by people, such as causing the imbalance of normal flora in the gastrointestinal tract of animals and producing drug resistance. The long-term use of antibiotics leads to a large amount of residues in meat and egg products in animal production. These residues are toxic, teratogenic, carcinogenic, and mutagenic to humans. Not only the quality of animal products is lowered, but the residual poisons damage the environment and pose a threat to the health of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K10/37A23K10/12A23K20/142A23K20/174A23K20/147
CPCY02P60/87
Inventor 潘宏涛卢亚萍
Owner 浙江伊杰斯生物科技有限公司