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Multi-dose melanin precursor substance hair dye and using method thereof

A technology of melanin precursors and hair dyes, which can be used in hair care, pharmaceutical formulations, cosmetic preparations, etc., and can solve problems such as environmental pollution, lack of pigment extraction technology, and long time consumption

Active Publication Date: 2019-04-23
珠海丝彩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide to hair dyes, and lack mature pigment extraction technology.
Known to have the method (patent document CN101164528A) of utilizing plant pigment catechol to prepare natural hair dye at present, but the catechol used wherein is to be extracted from catechin of Chinese medicinal material, and its shortcoming is that the planting scale of Chinese medicinal material is limited, and the pigment extraction cost Relatively high; Also have the report to utilize the catechol oxidase in the filamentous fungus cell of expressing mold to oxidize dopa and its derivatives by genetic engineering technology, convert it into the method of melanin precursor (patent document CN101914585A ), but the mold itself will produce some toxic and harmful substances, and the production process will cause environmental pollution. At the same time, there are disadvantages such as high cost, long time consumption, unstable performance, and inability to apply in large quantities.
In the prior art, there is also a method of using the enzyme liquid extracted from the Bacillus fermentation liquid to catalyze the substrate to produce melanin precursors, but the safety and non-toxic side effects of the product cannot be guaranteed, and the pollution in the fermentation process will also affect the product quality.

Method used

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  • Multi-dose melanin precursor substance hair dye and using method thereof
  • Multi-dose melanin precursor substance hair dye and using method thereof
  • Multi-dose melanin precursor substance hair dye and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] The preparation method of the melanin precursor of this embodiment is carried out according to the following steps:

[0133] 1. Extraction and determination of tyrosinase

[0134] Take 500 grams of the crushed Pleurotus eryngii and add 1000ml of distilled water to extract the tyrosinase solution or use 1000ml of neutral phosphate buffer to extract the tyrosinase solution.

[0135] The determination method of tyrosinase activity is as follows: take 0.1ml mushroom complex enzyme solution and 3.9ml substrate solution to react at 317nm for 10min, the enzyme activity unit U is defined as a change of 0.01 unit of absorbance per 1min as 1 enzyme activity unit. When the measured result is greater than 1000U, this enzyme solution can be used for the following melanin precursor accumulation reaction.

[0136] 2. Accumulation of melanin precursors

[0137] Prepare 2L of tyrosine solution with a concentration of 1mM-10mM, add the tyrosine substrate solution to the bioreactor with a volume ...

Embodiment 2

[0144] The preparation method of the melanin precursor of this embodiment is carried out according to the following steps:

[0145] 1. Extraction of Tyrosinase

[0146] The brown mushroom was crushed by a juice extractor, and 500 g of fruit body slag was added to 1000 ml of citric acid buffer solution with a pH of 6.8 to extract the enzyme solution, which was adsorbed by 5% activated carbon and centrifuged for use.

[0147] 2. Immobilization of Tyrosinase

[0148] Heat 200 mesh diatomaceous earth at 60°C for 30 minutes, then soak in phosphate buffer for 20 minutes, centrifuge to remove the supernatant, add the diatomaceous earth obtained after the treatment to the above enzyme solution and fix for 5 hours to obtain immobilized enzymes for use .

[0149] 3. Accumulation of melanin precursors

[0150] Prepare 2L of tyrosine solution with a concentration of 5mM, which can be completely dissolved by heating and dissolving.

[0151] Add the tyrosine substrate solution to a bioreactor with a v...

Embodiment 3

[0158] The preparation method of the melanin precursor of this embodiment is carried out according to the following steps:

[0159] 1. Extraction of Tyrosinase

[0160] Use a juice extractor to crush Agaricus bisporus, add 500g of the residue to 1000ml of Tris-hydrochloric acid buffer with a pH of 7.0 to extract the enzyme solution, centrifuge to extract the supernatant, and use 10% macroporous resin to adsorb polysaccharides and impurities in the enzyme solution. The treated enzyme solution is placed at 4°C for use.

[0161] 2. Accumulation of melanin precursors

[0162] Prepare 2L of tyrosine solution with a concentration of 10mM, which can be completely dissolved by heating and dissolving.

[0163] The reaction process is the same as in Example 1.

[0164] The liquid sample taken out was filtered through filter paper, and the filtrate was diluted 10 times, and the absorbance at 300 nm and 475 nm was measured in an ultraviolet spectrophotometer. When the absorbance at 300 nm is great...

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Abstract

The invention discloses a double-agent melanin precursor hair dye and a three-agent melanin precursor hair dye. The double-agent melanin precursor hair dye comprises an agent A and an agent B; the three-agent melanin precursor hair dye comprises an agent A, an agent B and an agent C; the agent A and the agent C both comprises melanin precursors, common hair dye auxiliaries and water; the common hair dye auxiliaries refer to one or more of a wetting agent, a thickening agent, a surfactant, a moisturizing agent and a pH value regulating agent. By combination of advantages of chemical synthesized hair dye and natural hair dye, compound enzyme liquor in natural edible fungi is extracted, amino acids are subjected to bioconversion to synthesize melanin precursor substances such as hallachrome, 5,6-dyhydroxyindole and 5,6-dyhydroxyindole-2-carboxylic acid, skin irritation and allergization are avoided, high safety is achieved, and natural black hair dyeing effects are kept. In addition, due to various agent type choices, the multi-agent melanin precursor hair dye has a great popularization and application value in the field of hair dye preparation.

Description

Technical field [0001] The invention relates to a preparation method for catalyzing and synthesizing a melanin precursor hair dye by extracting a biological composite enzyme, in particular to an extracting edible fungus composite enzyme solution, converting an amino acid substrate into a natural melanin precursor substance, and preparing it Into a non-oxidizing multi-dose biological hair dye. Background technique [0002] At present, as people pay more and more attention to health, the chemically synthesized hair dye contains carcinogenic and highly allergenic chemical dyeing components (such as phenylenediamines, aminophenols, etc.) and some harmful components (such as hydrogen peroxide). , Ammonia, etc.), has attracted more and more attention. For this reason, people try to seek natural harmless and safer hair dye materials. For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9728A61K8/66A61K8/44A61K8/49A61Q5/10
CPCA61K8/44A61K8/492A61K8/66A61K2800/88A61Q5/10
Inventor 张然薛俊厉秀茹
Owner 珠海丝彩生物科技有限公司
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