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Lactobacillus plantarum freeze-dried powder and preparation method thereof

A technology of Lactobacillus plantarum, freeze-dried powder, applied in biochemical equipment and methods, microorganism-based methods, bacteria and other directions, can solve problems such as poor freeze-drying protection effect, achieve good low-temperature preservation effect, and improve freeze-drying survival rate. Effect

Active Publication Date: 2016-10-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the poor freeze-drying protection effect of existing protective agents on Lactobacillus plantarum, and to provide a protective agent formula and preparation strategy suitable for Lactobacillus plantarum

Method used

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  • Lactobacillus plantarum freeze-dried powder and preparation method thereof
  • Lactobacillus plantarum freeze-dried powder and preparation method thereof
  • Lactobacillus plantarum freeze-dried powder and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The preparation of embodiment 1 lyoprotectant

[0026] Weigh a certain quality of WPI, dissolve it completely in a certain amount of purified water, prepare a WPI solution with a concentration of 100g / L-300g / L, and pasteurize it; then weigh a certain quality of inulin and sorbitol, and prepare two The concentrations of both are 80g / L-240g / L solution, sterilized at 115°C for 20 minutes; 40g / L-120g / L sodium ascorbate membrane filter to sterilize. After sterilization, each protective agent solution was mixed in a sterile environment at a volume ratio of 2:1:1 to obtain a freeze-dried protective agent.

Embodiment 2

[0027] The preparation of embodiment 2 Lactobacillus plantarum CCFM 8610 freeze-dried bacteria powder

[0028] (1) Composition of lyoprotectant: WPI 60g / L, sorbitol 40g / L, inulin 40g / L, sodium ascorbate 20g / L; conventional lyoprotectant composition: skimmed milk 120g / L, sucrose 20g / L, Trehalose 20g / L.

[0029] (2) Collection of bacterial cells: After the bacterial strains were cultivated to the end of the logarithmic growth phase, they were centrifuged at 8000g, the supernatant was discarded, washed twice with normal saline with pH 7.0, and finally the supernatant was discarded by centrifugation to obtain wet bacterial cells.

[0030] (3) Freeze-drying: take 0.5g, 1.0g, 1.5g, and 2.0g of wet thalline, add 10mL of the protective agent of the present invention and conventional protective agent respectively, after fully mixing, carry out vacuum freeze-drying, the freeze-drying steps are:

[0031] Fully mix the collected Lactobacillus plantarum and the freeze-drying protective ag...

Embodiment 3

[0051] The preparation of embodiment 3 Lactobacillus plantarum ATCC 14917 freeze-dried bacteria powder

[0052] (1) The lyoprotectant composition of the present invention: WPI 60g / L, sorbitol 40g / L, inulin 40g / L, sodium ascorbate 20g / L; conventional lyoprotectant composition: skimmed milk 120g / L, sucrose 20g / L, trehalose 20g / L.

[0053] (2) After the bacterial strain is cultivated to the end of the logarithmic growth phase, centrifuge at 8000g, discard the supernatant, wash twice with physiological saline with pH 7.0, and finally centrifuge and discard the supernatant to obtain wet cells.

[0054] (3) Freeze-drying: take 0.5g, 1.0g, 1.5g, and 2.0g of wet thalline, add 10mL of the protective agent of the present invention and conventional protective agent respectively, after fully mixing, carry out vacuum freeze-drying, the freeze-drying steps are:

[0055] Fully mix the collected Lactobacillus plantarum and the freeze-drying protective agent solution, pour the bacterial susp...

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Abstract

The invention discloses a lactobacillus plantarum freeze-dried powder and a preparation method thereof, and belongs to the field of production of biological bacteria products. The freeze-dried powder includes whey protein isolate, sorbitol, inulin, sodium ascorbate and lactobacillus plantarum, the content of WPI is 50 g / L-150 g / L, the content of sorbitol is 20 g / L-60 g / L, the content of inulin is 20 g / L-60 g / L, and the content of sodium ascorbate is 10 g / L-30 g / L. The freeze-dried powder significantly improves the survival rate of lactobacillus plantarum compared with a conventional protective agent; in the prepared lactobacillus plantarum freeze-dried powder, the freeze-dried survival rates of lactobacillus plantarum CCFM 8610, lactobacillus plantarum CCFM 8661 and other lactobacillus plantarum reach 100%.

Description

technical field [0001] The invention relates to a freeze-dried powder of Lactobacillus plantarum and a preparation method thereof, belonging to the production field of biological bacteria products. Background technique [0002] Lactobacillus plantarum has attracted extensive attention and been deeply researched and developed because of its superior fermentation performance and diverse functions. At present, Lactobacillus plantarum has been widely used in nutrition and health care, fermented food, biomedicine, feed, animal husbandry and other industries. Lactobacillus plantarum products such as kimchi direct-throwing starter, lactic acid bacteria tablets, and active lactic acid bacteria beverages are deeply loved by consumers. However, Lactobacillus plantarum has low resistance to the external environment, and the imperfect domestic production technology leads to low survival rate of bacteria powder cells. Ensuring no loss of Lactobacillus plantarum is the key technology to ...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/20C12R1/25
CPCC12N1/04C12N1/20
Inventor 陈卫崔树茂翟齐啸赵建新田丰伟王刚张灏
Owner JIANGNAN UNIV
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