Bacterial strain capable of realizing high yield of L-alanine

Abstract

The invention provides a bacterial strain capable of realizing high yield of L-alanine, belonging to the field of bioengineering technology. The bacterial strain capable of realizing high yield of L-alanine is named as Escherichia coli (EcQLS1) with an accession number of CGMCC No. 1764. The Escherichia coli (EcQLS1) strain is obtained through a combination of normal-temperature normal-pressure plasma mutation and high-flux screening, can greatly improve the yield of L-alanine and has good stability, wherein the yield of L-alanine has no obvious change after serial passage of the strain for five generations. Results of shake-flask experiments show that the yield of L-alanine reaches 120 g / L. The bacterial strain provided by the invention totally meets industrial mutation and screening demands on an L-alanine strain, is applicable to industrial fermentation production and has significant economic value.

Description

technical field

[0001] The invention belongs to the technical field of bioengineering and provides an L-alanine high-yield bacterial strain. Background technique

[0002] L-alanine, also known as α-alanine, is a left-handed isomer of α-amino acid, which is a white crystal with a special sweet taste. It is widely used in the fields of biology, medicine, chemical industry, food, polymer materials, etc., for the preparation of pharmaceutical compounds, synthetic sweeteners, and determination of liver function. It is an important chemical product with a wide range of applications and great development potential. More and more more popular and valued by people.

[0003] Currently, L-alanine can be produced by chemical synthesis, enzymatic and fermentation methods. It is a common method in the chemical industry to produce various amino acids through different chemical synthesis routes, but the synthetic method has poor product quality, low recovery rate, high cost and environmen...

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