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Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof

A technology of immunochromatography test paper and immunochromatography, which is applied in the field of clinical hematology detection, to achieve the effects of strong specificity, protection of blood resources, and saving of blood resources

Inactive Publication Date: 2016-10-26
诺莱生物医学研究院(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention is the first in China, and there is no immune colloidal gold test strip for rapid detection of chylotemia in the market

Method used

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  • Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof
  • Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof
  • Immunochromatographic test paper card for rapid detection of chylemia and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] see figure 1 and figure 2 , a kind of immunochromatographic test paper card for rapid detection of chyle blood of the present invention comprises a colloidal gold test paper strip A and a gusset type card slot 8, and the gusset type card slot 8 has a built-in cavity for accommodating the colloidal gold test paper strip A Cavity 11, and observation port 9 and sampling hole 10 are arranged on this gusset type draw-in groove 8; Water-absorbing pad 6 is composed of, on one side of the support plate 7, the sample pad 1, the gold-labeled antibody pad 2, the NC film 3 and the water-absorbing pad 6 are sequentially bonded from one end to the other end; A detection line 4 of the polyclonal antibody of apolipoprotein B and a quality control line 5 containing IgG secondary antibody, the detection line 4 and the quality control line 5 are arranged at intervals, and the detection line 4 is adjacent to the gold-labeled antibody pad 2; the colloid The gold test strip A is set in th...

Embodiment 2

[0039] The preparation method of the test paper card for rapid detection of chyle blood comprises the following steps:

[0040] 1) Preparation of NC membrane 3

[0041] Preparation of detection line 4: using a gold spraying instrument, dilute the carrier protein B polyclonal antibody with coating buffer, draw a line at the position of the detection line in the NC membrane, which is the detection line, and dry it for later use; the coating buffer 0.05M pH9.6 carbonate buffer filtered through a 0.45μm filter membrane;

[0042] Preparation of quality control line 5: use a gold spraying instrument, dilute the rabbit anti-mouse IgG secondary antibody with coating buffer, draw a line on the NC membrane at a position parallel to the detection line, with an interval of 5 mm, which is the quality control line. The coating buffer is 0.05 M pH9.6 carbonate buffer filtered through a 0.45 μm filter membrane;

[0043] NC film assembly and preparation: dry the test line and quality control...

Embodiment 3

[0049] After preparing the chyle blood rapid detection test paper card according to the method of Example 1 and Example 2, it was used according to the following method.

[0050] 1) The use and judgment of the rapid test paper card for chyle blood

[0051] When performing primary screening on blood donors who donate blood on the street, collect 40 microliters of terminal blood from the blood donors and drop them on the sample pad 1 of the test paper card ( figure 1 middle arrow B), after adding blood, the serum will press figure 1 The direction indicated by the middle arrow C is siphoned forward. The apoB48 in the serum will first specifically bind to the apoB48 monoclonal antibody labeled with colloidal gold on the gold label pad 2, and move forward with the serum. When it reaches the position of the detection line 4, ApoB48 will be captured by the apoB48 polyclonal antibody immobilized on detection line 4, and when enough apoB48 is captured, the colloidal gold attached to i...

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Abstract

The invention relates to an immunochromatographic test paper card for rapid detection of chylemia and a preparation method thereof. The detection test paper card comprises a colloidal gold test strip and a card slot, wherein the colloidal gold test strip mainly comprises a support plate, an NC film, a gold-labeled antibody pad, a sample pad and a water absorbing pad, and the NC film is arranged in the middle of the support plate; the gold-labeled antibody pad and the sample pad are pasted on the support plate at one side of the NC plate, and the water absorbing pad is pasted on the support plate at the other side of the NC plate; a detection line containing a polyclonal antibody of apoB and a quality control line containing a secondary antibody are arranged in the middle of the NC film. The test paper card has the advantages of rapid detection, high accuracy, strong specificity and the like, does not need to use an instrument and equipment, and is convenient to use. The test paper card for rapid detection of chylemia prepared by the invention can rapidly screen chylemia for blood donors before blood donation, thereby protecting the blood resources provided by the blood donors to a great extent, and also saving the relevant detection expense and the like.

Description

technical field [0001] The invention belongs to clinical hematology detection, in particular to a colloidal gold test paper card for rapid detection of chyle blood and a preparation method thereof. Background technique [0002] Chylotemia or lipemia, the postprandial fat is digested and absorbed in the small intestine, and becomes fine chylomicrons and enters the blood. When there are a certain amount of chylomicrons, the serum changes from a clear and transparent light yellow liquid to a milky white viscous liquid . Plasma cannot be infused to patients due to the high triglyceride content. Triglycerides are an independent risk factor for cardiovascular disease. At present, there is no method in China to quickly detect triglyceride levels in peripheral blood when collecting blood on the street. Therefore cause a large amount of fat blood to be scrapped every year, cause the huge waste of blood resource. [0003] Apolipoprotein (apolipoprotein, apo) is a protein in plasma...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 王成刘建强
Owner 诺莱生物医学研究院(北京)有限公司
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